论文标题：Lysine benzoylation is a histone mark regulated by SIRT2

期刊：Nature Communications

作者：Yingming Zhao et al

发表时间： 2018/08/28

数字识别码：10.1038/s41467-018-05567-w

原文链接：https://www.nature.com/articles/s41467-018-05567-w?utm_source=Other_website&utm_medium=Website_links&utm_content=RenLi-MixedBrand-OAB-Multidisciplinary-China&utm_campaign=OAB_USG_JRCN_RL_article_promotion_sciencenet_Aug_5th

微信链接：https://mp.weixin.qq.com/s/vtWR8VAyS1Beq-gUe9G7fg

《自然-通讯》近日发表的一项研究Lysine benzoylation is a histone mark regulated by SIRT2表明，食品防腐剂苯甲酸钠能通过培养细胞融入其表观基因组——表观基因组指基因组中调控基因表达的一系列标记物。该研究结果提出了一种能将膳食成分整合到细胞内的潜在机制。不过，食用苯甲酸钠的生物体内能否检测到类似结果仍待进一步研究。

图1：来源：Pixabay

越来越多的证据表明，饮食不仅能影响我们的身体健康和预期寿命，还可能帮助我们预防特定疾病，如肥胖、糖尿病、癌症和精神疾病。不过，饮食对人体生理机能的影响机制尚不明确。实现细胞功能修饰的一种可能方式是通过表观遗传调控。

图2：赖氨酸乙酰化和苯甲酰化。图源：Huang et al.

美国芝加哥大学的Yingming Zhao及同事运用蛋白质组学和生物化学方法，在体外培养的细胞中发现了赖氨酸苯甲酰化（lysine benzoylation）这种新型组蛋白翻译后修饰。作者发现，这种组蛋白标记的沉积会影响细胞的基因表达，从而影响各种代谢相关通路，如胰岛素的分泌。作者认为苯甲酸钠是赖氨酸苯甲酰化修饰底物的一大来源，且苯甲酸钠能促进这种组蛋白标记的累积。

摘要：Metabolic regulation of histone marks is associated with diverse biological processes through dynamically modulating chromatin structure and functions. Here we report the identification and characterization of a histone mark, lysine benzoylation (Kbz). Our study identifies 22 Kbz sites on histones from HepG2 and RAW cells. This type of histone mark can be stimulated by sodium benzoate (SB), an FDA-approved drug and a widely used chemical food preservative, via generation of benzoyl CoA. By ChIP-seq and RNA-seq analysis, we demonstrate that histone Kbz marks are associated with gene expression and have physiological relevance distinct from histone acetylation. In addition, we demonstrate that SIRT2, a NAD+-dependent protein deacetylase, removes histone Kbz both in vitro and in vivo. This study therefore reveals a new type of histone marks with potential physiological relevance and identifies possible non-canonical functions of a widely used chemical food preservative.

阅读论文全文请访问：https://www.nature.com/articles/s41467-018-05567-w?utm_source=Other_website&utm_medium=Website_links&utm_content=RenLi-MixedBrand-OAB-Multidisciplinary-China&utm_campaign=OAB_USG_JRCN_RL_article_promotion_sciencenet_Aug_5th

（来源：科学网）