来源:Frontiers of Environmental Science & Engineering 发布时间:2022/4/29 13:18:20
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FESE | 前沿研究:紫外/氯处理对再生水中微生物群落结构、典型病原菌及耐药基因的影响

论文标题:Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes in reclaimed water. (紫外/氯处理对再生水中微生物群落结构、典型病原菌及耐药基因的影响)

期刊:Frontiers of Environmental Science & Engineering

作者:Chengsong Ye (叶成松) , Yuming Chen (陈煜铭) , Lin Feng (冯琳) , Kun Wan (万堃) , Jianguo Li (李建国) , Mingbao Feng (冯明宝) , Xin Yu (于鑫)

发表时间:09 Dec 2021

DOI:10.1007/s11783-022-1521-z

微信链接:点击此处阅读微信文章

原文链接:

https://journal.hep.com.cn/fese/EN/10.1007/s11783-022-1521-z

文章出版:Front. Environ. Sci. Eng. 2022, 16(8): 100

原文信息

题目:

Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes in reclaimed water

作者:

Chengsong Ye (叶成松) 1, Yuming Chen (陈煜铭) 2, Lin Feng (冯琳) 3, Kun Wan (万堃) 1, Jianguo Li (李建国) 1, Mingbao Feng (冯明宝) 1, Xin Yu (于鑫) () 1

作者单位:

1 Xiamen University, China

2 Xiamen Foreign Language School, China

3 Renmin University of China, China

通讯作者邮箱:

xyu@xmu.edu.cn

关键词:

UV/chlorine process (紫外/氯);

Pathogen (病原菌);

Antibiotic resistance genes (耐药基因);

High-throughput qPCR (高通量定量PCR);

Reclaimed water (再生水)

文章亮点

• 紫外/氯处理可有效去除再生水中的VBNC病原菌、ARGs和MGEs;

• 紫外/氯处理后,再生水中微生物群落多样性降低;

• 紫外/氯处理后,携带MGEs的耐氯菌成为优势群;

• 紫外/氯对于细菌的抗性组没有直接选择性。

文章简介

污水回用是解决水资源短缺问题的重要途径。以往的研究表明,目前污水处理厂并不能完全去除新型污染物,如活的不可培养状态(VBNC)型病原菌和抗生素耐药基因(ARGs)。这些污染物由于其环境耐受性和传播潜力而成为再生水安全评价中值得关注的问题。

本研究采用紫外/氯处理实际再生水样品,评估体系中典型病原菌、VBNC型菌以及细菌群落的变化。此外,通过高通量定量PCR技术,系统的研究了ARGs在紫外/氯处理后的赋存规律。研究认为紫外/氯处理可以潜在地提高再生水的微生物安全性,具体结论如下:

(1)紫外/氯处理可有效去除再生水中的可培养细菌。然而,去除VBNC型病原菌需要更长的反应时间。同时,水质理化指标不受紫外/氯的影响;

(2)典型病原菌在紫外/氯处理后,其绝对丰度显著降低,但相对丰度增加,且无季节性规律。不动杆菌和分枝杆菌是群落中主要的细菌类型,两者都是tnpA基因的潜在宿主。因此,对这类携带MGEs的耐氯致病菌应重点关注;

(3)细菌总数和水质是影响紫外/氯处理效率的两个重要因素,它们主要是通过加速氯的衰减过程,即余氯不足导致自由基生成低,无法保证协同消毒效果;

(4)随着紫外/氯的剂量增加,ARG和MGEs的数量和绝对丰度均降低。然而,在紫外/氯处理后,有11个基因在超过3个季节中仍被检出(持久性基因),这些基因含有高比例的MGEs,可能会增加ARG水平转移的频率。

图1 摘要图

编者点评

水资源的短缺增加了污水回用的需求,再生水中的病原菌和耐药基因给用水健康带来隐患。本研究以实际再生水为例,探索了紫外/氯高级氧化技术对其群落结构、典型病原菌和ARG的影响,研究认为紫外/氯处理可以潜在地提高再生水的微生物安全性,但携带MGE的耐氯病原菌依旧是消毒的难点。

编者简介

叶成松,男,厦门大学环境与生态学院2019级博士生,导师为于鑫教授,研究方向为水环境中微生物的行为及控制技术。

摘要

Urban wastewater contains a wide range of pathogens and antibiotic resistance genes (ARGs), which are a serious concern if reusing treated wastewater. However, few studies have explored the microbial communities in reclaimed water using ultraviolet (UV)/chlorine treatment and assessed the changes of the resistome. This study investigated the occurrence of typical pathogens, ARGs, and bacterial communities in UV/chlorine-treated reclaimed water samples. The numbers of culturable and viable but non-culturable pathogens were effectively reduced to 0 CFU/mL within 1–10 and 10–30 min after UV/chlorine treatment, respectively. Meanwhile, the physicochemical indices of water quality were not affected. UV/chlorine treatment could significantly change the bacterial community structure of reclaimed water, showing a decrease in bacterial abundance and diversity. Chlorine-resistant Acinetobacter and Mycobacterium were the dominant bacterial genera (>50%) after UV/chlorine treatment. Moreover, the number of ARGs and mobile genetic elements (MGEs) decreased with an increase in UV/chlorine exposure. However, eight ARGs and three MGEs were consistently detected in more than three seasons, making these major concerns because of their potential role in the persistence and dissemination of antibiotic resistance. Overall, the results of this study suggest that UV/chlorine treatment can potentially improve the microbiological safety of reclaimed water. And more attention should be paid to the pathogens that are both chlorine-resistant and carry MGEs because of their potential for resistance transmission.

《前沿》系列英文学术期刊

由教育部主管、高等教育出版社主办的《前沿》(Frontiers)系列英文学术期刊,于2006年正式创刊,以网络版和印刷版向全球发行。系列期刊包括基础科学、生命科学、工程技术和人文社会科学四个主题,是我国覆盖学科最广泛的英文学术期刊群,其中13种被SCI收录,其他也被A&HCI、Ei、MEDLINE或相应学科国际权威检索系统收录,具有一定的国际学术影响力。系列期刊采用在线优先出版方式,保证文章以最快速度发表。

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