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基于CRISPR技术的策略或可消除大蕉的香蕉条纹病|《通讯-生物学》论文 |
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论文标题:CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding
期刊:Communications Biology
作者:Jaindra N. Tripathi, Valentine O. Ntui, Mily Ron, Samwel K. Muiruri, Anne Britt, Leena Tripathi
发表时间:2019/01/31
数字识别码: 10.1038/s42003-019-0288-7
原文链接:http://t.cn/EVhfVlw
微信链接:https://mp.weixin.qq.com/s/ZkUNS0DVEdOSJfnrq0zw3g
本周《通讯-生物学》在线发表的一项研究CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding报告了一种可以成功消除大蕉的香蕉条纹病毒的策略。这是一种基于CRISPR技术的策略,有望帮助改善大蕉的生长,并提高其产量。
图1:香蕉条纹病毒Obino lEwai(BSOLV)的示意图和Gonja Manjaya植物的分子分析
图源:Tripathi等
香蕉及其近亲大蕉是热带和亚热带国家的一种重要的主食类作物。培育具有更高的抗病抗虫特性的品种是保证其健康高产的关键。香蕉条纹病毒是一种分布广泛的病毒,它通过将自身的DNA插入香蕉B基因组而致病,最终可导致作物死亡。
比如,当香蕉受到干旱或高温威胁时,香蕉条纹病毒DNA会产生功能性病毒颗粒,最终引发疾病症状。因此,培育者在改良香蕉时会避免使用包含B基因组的香蕉,比如野蕉(Musa balbisiana),尽管野蕉具有一些优良特性,包括抗寒性、根系发达和抗逆性。
图2:通过农杆菌介导的Gonja Manjaya细胞悬液转化来递送CRISPR / Cas9系统,从而产生基因组编辑事件。 图源:Tripathi等
肯尼亚国际热带农业研究所(IITA)的Jaindra Tripathi及同事使用CRISPR/Cas9系统使Gonja Manjaya B基因组中的病毒DNA失活。Gonja Manjaya是英文俗名为false horn的一类大蕉中的一种,主要生长在非洲中东部。作者发现,暴露在干旱压力下时,与未经编辑的大蕉相比,经过编辑的大蕉有75%没有展现出香蕉条纹病毒的症状,证明病毒DNA确实失活。
图3:评估编辑过的和野生型未经编辑对照组的Gonja Manjaya在水分胁迫条件下诱导的香蕉条纹病毒症状。图源:Tripathi等
作者总结表示,这种策略或可用于改良香蕉和大蕉,开发具有强化B基因组的新杂交品种。
摘要:Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated.
阅读论文全文请访问:http://t.cn/EVhfVlw
期刊介绍:Communications Biology (https://www.nature.com/commsbio/) is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the biological sciences. Research papers published by the journal represent significant advances bringing new biological insight to a specialized area of research.
(来源:科学网)
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