来源:Scientific Reports 发布时间:2019/5/28 19:22:57
利用硅芯片技术实现更快的犯罪现场DNA鉴定|Scientific Reports

论文标题:Silicon µPCR Chip for Forensic STR Profiling with Hybeacon Probe Melting Curves

期刊:Scientific Reports

作者:Senne Cornelis, Olivier Tytgat, Maarten Fauvart, Yannick Gansemans, Ann-Sophie Vander Plaetsen, Rodrigo S. Wiederkehr, Dieter Deforce, Filip Van Nieuwerburgh, Tim Stakenborg


数字识别码: 10.1038/s41598-019-43946-5




来自比利时根特大学的Dieter Deforce及同事在近期发表于Scientific Reports的一项研究Silicon µPCR Chip for Forensic STR Profiling with Hybeacon Probe Melting Curves中展示了一种利用单片微单晶的技术,该技术有望替代DNA指纹分析法。这项技术将PCR(Polymerase Chain Reaction,聚合酶链反应)扩增技术与硅芯片实验室(LoC, Lab-on-a-chip)中的HyBeacon熔融分析相结合,在快速现场DNA指纹图谱鉴定领域迈出了重要的一步。


LoC形体小巧,因而降低了试剂消耗并且增加了便携性。LoC的小型化优势是通过一个集成加热元件实现的,这个集成元件包含24个平行微型反应器,每个反应器的反应体积为0.14L。而高度的平行化使得作者可以同时分析4个短串联重复序列(STR,short tandem repeat)位点以及法医学DNA分析中常见的釉原蛋白性别标记。


关于Scientific Reports

•         Scientifi­c Reports articles attracted 196,000+ citations in 2017 - the 16th most cited journal in the world in 2017

•         High Visibility with 5 million page views per month, 65,000+ mentions in news, cited by 190+ policy documents including reports from the World Bank, WHO, CDC and GOV.UK

•         Journal Matrix: Eigenfactor: 0.71896, Article In­fluence score: 1.356, Impact Factor: 4.122


摘要:The demand to perform forensic DNA profiling outside of centralized laboratories and on the crime scene is increasing. Several criminal investigations would benefit tremendously from having DNA based information available in the first hours rather than days or weeks. However, due to the complexity and time-consuming nature of standard DNA fingerprinting methods, rapid and automated analyses are hard to achieve. We here demonstrate the implementation of an alternative DNA fingerprinting method in a single microchip. By combining PCR amplification and HyBeacon melting assays in a silicon Lab-on-a-chip (LoC), a significant step towards rapid on-site DNA fingerprinting is taken. The small form factor of a LoC reduces reagent consumption and increases portability. Additional miniaturization is achieved through an integrated heating element covering 24 parallel micro-reactors with a reaction volume of 0.14 µl each. The high level of parallelization allows the simultaneous analysis of 4 short tandem repeat (STR) loci and the amelogenin gender marker commonly included in forensic DNA analysis. A reference and crime scene sample can be analyzed simultaneously for direct comparison. Importantly, by using industry-standard semiconductor manufacturing processes, mass manufacturability can be guaranteed. Following assay design and optimization, complete 5-loci profiles could be robustly generated on-chip that are on par with those obtained using conventional benchtop real-time PCR thermal cyclers. Together, our results are an important step towards the development of commercial, mass-produced, portable devices for on-site testing in forensic DNA analysis.


期刊介绍:Scientific Reports (https://www.nature.com/srep/) is an online, open access journal from the publishers of Nature. We publish scientifically valid primary research from all areas of the natural and clinical sciences.

The 2017 journal metrics for Scientific Reports are as follows:

•2-year impact factor: 4.122

•5-year impact factor: 4.609

•Immediacy index: 0.576

•Eigenfactor® score: 0.71896

•Article Influence Score: 1.356

•2-year Median: 2


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