中国药科大学药学院龙燕等合作的一项最新研究，提出了一个前边缘皮层-下丘脑外侧回路调节阿普唑仑诱导的雄性小鼠条件位置偏好的获得。该研究于2026年8月19日发表于《中国药理学报》杂志上。

研究人员第一次建立Alp管理是在10点mg/kg可靠诱导雄性小鼠条件位置偏好（CPP）。结果表明，alp训练小鼠的前边缘皮质（PrL）CaMKⅡα神经元过度活跃。PrL CaMKⅡα神经元活性的化学发生调控双向获得alp诱导的CPP。

此外，virthem示踪显示PrL CaMKⅡα神经元向外侧下丘脑（LH）强烈投射。抑制PrL-LH回路可显著降低alp诱导的CPP的获得，而增强该回路可促进alp诱导的CPP。激活PrLCaMKⅡα-LH电路不能产生实时位置偏好。综上所述，他们的研究结果表明，PrL谷氨酸能神经元和PrL- lh回路调节了Alp诱导的奖励记忆形成，为阐明Alp成瘾的神经机制提供了基础。

研究人员表示，阿普唑仑（Alprazolam，简称Alp）是一种高效苯二氮卓类药物，具有显著的精神失常和成瘾风险，但与Alp相关的奖励记忆的确切机制尚不明确。

附：英文原文

Title: A prelimbic cortex-lateral hypothalamus circuit regulates acquisition of alprazolam-induced conditioned place preference in male mice

Author: Song, Zhi-peng, Li, Nian-ci, Song, Rui, Zheng, Cheng, Wang, Cun, Liao, Fei-zhi, Tang, Su-su, Yuan, Dan-hua, Hong, Hao, Chen, Zhi-gang, Long, Yan

Issue&Volume: 2026-08-19

Abstract: Alprazolam (Alp), a high-potency benzodiazepine, poses a significant risk of abuse and addiction, yet the precise mechanisms underlying Alp-associated reward memory remain poorly defined. Here, we first established that administration of Alp at 10mg/kg reliably induces conditioned place preference (CPP) in male mice. Our results indicated that prelimbic cortex (PrL) CaMKⅡα neurons were hyperactive in Alp-trained mice. Chemogenetic manipulation of the activity of PrL CaMKⅡα neurons bidirectionally regulated acquisition of Alp-induced CPP. Furthermore, virus tracing indicated a strong PrL CaMKⅡα neuron projection to lateral hypothalamus (LH). Suppression of the PrL-LH circuit significantly decreased acquisition of Alp-induced CPP, while potentiation of this circuit facilitated Alp-induced CPP. Activation of PrLCaMKⅡα-LH circuit failed to produce real-time place preference. In summary, our results indicate that PrL glutamatergic neurons and the PrL-LH circuit regulate Alp-induced reward memory formation, providing a foundation for elucidating the neural mechanisms underlying Alp addiction.

DOI: 10.1038/s41401-026-01909-3

Source: https://www.nature.com/articles/s41401-026-01909-3