华东理工大学朱麟勇小组宣布他们提出了NirFAP680：一种高亮度、稳定的大斯托克斯位移近红外荧光激活蛋白。相关论文发表在2026年4月21日出版的《自然—方法学》杂志上。

文章介绍了NirFAP680的研制过程；NirFAP680是一种近红外荧光激活蛋白（FAP），由一种新设计的近红外荧光剂HBMT和一种工程蛋白标签NirFAP组成。在单光子和双光子激发下，与目前可用的近红外FAPs和荧光蛋白相比，NirFAP680具有更高的细胞亮度和优越的光稳定性，并允许对活细胞和体内的蛋白质进行自动成像。得益于其斯托克斯位移超过100 nm的独特光谱特性，NirFAP680还可以作为荧光或生物发光共振能量转移的极好受体，分别可以实时监测活细胞中蛋白质-蛋白质相互作用和体内灵敏的生物发光成像。因此，NirFAP680可能会成为活细胞和体内高级生物成像的主题。

据介绍，近红外（NIR）基因编码荧光标签优越的新成像能力，从多路成像到深层组织成像。

附：英文原文

Title: NirFAP680: a highly bright and stable large Stokes shift near-infrared fluorogen-activating protein

Author: Chen, Zhengda, Jiang, Li, Liu, Xiaoyu, Shi, Yajie, Liu, Yang, Jiang, Ling, Li, Jiaxin, Huang, Xinyi, Zhou, Siyu, Bao, Bingkun, Zhang, Bibi, Lin, Qiuning, Bian, Qi, Yin, Yuxin, Zuo, Fangting, Su, Ni, Tang, Yun, Zhao, Yuzheng, Yang, Yi, Chen, Xianjun, Zhu, Linyong

Issue&Volume: 2026-04-21

Abstract: Near-infrared (NIR) genetically encoded fluorescent tags are superior for new imaging capabilities ranging from multiplexed imaging to deep-tissue imaging. Here, we describe the development of NirFAP680; NirFAP680 is an NIR fluorogen-activating protein (FAP) that consists of a newly designed NIR fluorogen termed HBMT and an engineered protein tag termed NirFAP. NirFAP680 has an order of magnitude greater cellular brightness and superior photostability compared to currently available NIR FAPs and fluorescent proteins in both single- and two-photon excitation and allows robust imaging of proteins in live cells and in vivo. Owing to its unique spectroscopic property of a >100nm Stokes shift, NirFAP680 can also serve as a superb receptor for fluorescence or bioluminescence resonance energy transfer, allowing real-time monitoring of protein–protein interactions in live cells and sensitive bioluminescence imaging in vivo, respectively. Therefore, NirFAP680 will likely be useful for advanced biological imaging in live cells and in vivo.

DOI: 10.1038/s41592-026-03061-6

Source: https://www.nature.com/articles/s41592-026-03061-6