研究组报告了睾丸体细胞的重组主题多能干细胞。重组重现了性别决定的过程，产生了形成半精小管和相邻间质组织的细胞类型。重建的睾丸组织整合了多能干细胞衍生的原始生殖细胞，并支持其向精原干细胞分化。这些精原干细胞在移植到睾丸后分化为功能精子。本研究有助于加深对性别决定过程的理解，并有助于在培养中为男性生殖系创造一种替代的环境。
据介绍，性腺体细胞的适当分化对性别决定和性激素和配子的产生至关重要，在培养中重建这一过程既加深了他们对这一过程的理解，又使体外配子的产生成为可能。
附：英文原文
Title: Reconstitution of sex determination and the testicular niche using mouse pluripotent stem cells
Author: Takashi Yoshino, Hiromichi Sasada, Takuya Sato, Tomonori Nakamura, Kenjiro Shirane, Hiroshi Ohta, Maki Kamoshita, Miki Inoue, Yuki Matsudaira, Chao Liu, Minatsu Matsufuji, Makoto Tachibana, Ken-Ichirou Morohashi, Masahito Ikawa, Mitinori Saitou, Takehiko Ogawa, Katsuhiko Hayashi
Issue&Volume: 2026-02-26
Abstract: Proper differentiation of gonadal somatic cells is crucial for sex determination and the production of sex hormones and gametes, and reconstituting this process in culture would both deepen our understanding of this process and enable the generation of gametes in vitro. Here, we report the reconstitution of testicular somatic cells using mouse pluripotent stem cells. The reconstitution recapitulated the sex-determination process, yielding cell types that formed seminiferous tubules and adjacent interstitial tissues. The reconstituted testicular tissue incorporated pluripotent stem cell–derived primordial germ cells and supported their differentiation into spermatogonial stem cells. These spermatogonial stem cells differentiated into functional spermatozoa upon transplantation into testis. This study contributes to a deeper understanding of the sex-determination process and to the creation of an alternative source for the male germ line in culture.
DOI: aea0296
Source: https://www.science.org/doi/10.1126/science.aea0296