近日，北京大学深圳研究生院彭涛团队研究了可激活体内成像的二羟蒽衍生二氧乙烷化学发光团。相关论文于2025年9月2日发表在《德国应用化学》杂志上。

化学发光团具有最佳的化学发光特性和适应可激活化学发光探针的多用途结构，由于其高灵敏度和信噪比，在生物传感和成像中特别受关注。然而，这种化学发光团仍然是有限的。

研究组报道了二羟蒽衍生的化学发光团（DhX-CLs），它将金刚烷二氧基二氧乙烷部分与合理设计的二羟蒽类核心骨架结合在一起，具有扩展和限制π电子系统。对基于二羟蒽醛的原型化学发光团进行结构优化，在水条件下生成了亮黄色的化学发光团DhX-CL4，其量子产率为35.4%，在500 nm以上的绿-近红外（NIR）范围内高于现有的二氧乙烷。进一步进行π-电子延伸和受体工程，得到了具有高亮度和生物相容性的近红外化学发光基团。

值得注意的是，DhX-CL7具有持久的化学发光，最大近红外发射波长为808 nm，量子产率为3.17%，高于大多数报道的近红外化学发光团。研究组进一步证明，这些化学发光团与分析响应部分的笼化提供了可激活的化学发光探针，用于β-半乳糖苷酶和过氧亚硝酸盐的体内成像。总的来说，这个新的化学发光团家族具有可调谐的发射波长和显著的化学发光特性，可作为一个多功能的体内化学发光成像平台。

附：英文原文

Title: Dihydroxanthene-Derived Dioxetane Chemiluminophores for Activatable in Vivo Imaging

Author: Kongke Liao, Lina Zhang, Jie Jiang, Feifei Wang, Tao Peng

Issue&Volume: 2025-09-01

Abstract: Chemiluminophores with optimal chemiluminescence properties and versatile structures adaptable into activatable chemiluminescent probes are of particular interest for biosensing and imaging owing to their high sensitivity and signal-to-noise ratio. However, such chemiluminophores are still limited. Herein, we report dihydroxanthene-derived chemiluminophores (DhX-CLs) that integrate an adamantyl-dioxetane moiety with a rationally designed dihydroxanthene-like core skeleton featuring an extended and restricted π-electron system. Structural optimization of the dihydroxanthene-aldehyde-based prototype chemiluminophore generated a bright yellow chemiluminophore DhX-CL4 with a quantum yield of 35.4% under aqueous conditions, higher than those of existing dioxetanes in the green-to-near-infrared (NIR) range beyond 500 nm. Further π-electron extension and acceptor engineering resulted in NIR chemiluminophores with high brightness and biocompatibility. Notably, DhX-CL7 exhibits long-lasting chemiluminescence with a maximum NIR emission at 808 nm and a quantum yield 3.17%, higher than those of most reported NIR chemiluminophores. We further demonstrated that caging of these chemiluminophores with analyte-responsive moieties afforded activatable chemiluminescent probes for in vivo imaging of β-galactosidase and peroxynitrite. Overall, this new family of chemiluminophores, with tunable emission wavelengths and remarkable chemiluminescence properties, serves as a versatile platform for in vivo chemiluminescence imaging.

DOI: 10.1002/anie.202514236

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202514236