近日，大连理工大学孙文团队实现了高细胞活性循环稀有细胞的可重复利用底物捕获和红光响应释放。2025年9月15日出版的《德国应用化学》杂志发表了这项成果。

高效和可替换的生物医学界面是分离和无损释放血液中循环的稀有细胞所必需的。然而，光响应释放策略，特别是主题红光，依赖于光热效应，这可能会通过局部过热影响细胞活性。

为了克服这个问题，研究组开发了基于钌（Ru）配合物的可重复使用的功能性底物（RuSub-S-SA-Ab），该配合物具有光响应配体替代，通过使用具有高灵敏度和特异性的可替代抗体来捕获细胞。该RuSub-S-SA-Ab在四种不同的细胞系中实现了>85%的捕获效率，在安全剂量的红光（660 nm，100 mW·cm^-2，60 S）下实现了90%的释放效率，从而为开发具有高生物安全性的响应式生物医学界面提供了一个新平台。

附：英文原文

Title: A Reusable Substrate for Capture and Red Light-Responsive Release of Circulating Rare Cells with High Cytoactivity

Author: Zehou Su, Ran Wang, Dongmei Xi, Jiexuan Zuo, Zongwei Zhang, Wenkai Liu, Xiaolong Zeng, Jianjun Du, Jiangli Fan, Xiaojun Peng, Wen Sun

Issue&Volume: 2025-09-15

Abstract: Efficient and reusable biomedical interfaces are necessary for the isolation and nondestructive release of circulating rare cells in blood. However, photo-responsive release strategy, particularly that using red light, relies on photothermal effect, which could potentially affect cell activity through local overheating. To overcome this issue, we develop reusable functional substrates (RuSub-S-SA-Ab) based on ruthenium (Ru) complexes with photo-responsive ligand substitution to capture cells by using replaceable antibodies with high sensitivity and specificity. Our RuSub-S-SA-Ab achieved capture efficiencies >85% for four different cell lines and release efficiencies of 90% under safe doses of red light (660 nm, 100 mW·cm2, 60 s), thus offering a new platform for the development of responsive biomedical interfaces with high biosafety.

DOI: 10.1002/anie.202516070

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202516070