近日，复旦大学张凡团队研究了用于实时体内多光谱成像的激发编码单发射短波红外镧系荧光团调色板。这一研究成果于2025年8月27日发表在《自然—光子学》杂志上。

多路荧光成像提供了从细胞到组织水平的有价值的生物学见解，但由于缺乏能够克服光子散射和自身荧光噪声的荧光调色板，因此在活体哺乳动物研究中用于实时、多路体内成像仍然受到限制，

研究组提出了一种由铒(III)-酞菁配合物设计的荧光团调色板，称为镧系彩虹（Lanbow），它提供可调的近红外吸收和统一的1,530纳米发光，亮度超过现有的分子染料。Lanbow主题兴奋编码和高效单波段检测在1500~1900纳米短波红外亚区，组织散射和自身荧光被最小化，在深层组织中实现多达九色成像。研究组还演示了荧光引导手术的多参数解剖识别和功能评估，并使用深度学习网络自动实时分析术中指导。这项研究为活体哺乳动物的实时、高复用成像建立了一个变革性的平台。

附：英文原文

Title: Excitation-encoded single-emission shortwave infrared lanthanide fluorophore palette for real-time in vivo multispectral imaging

Author: Zhang, Lu, Cheng, Ri, He, Zuyang, Mei, Mei, Wu, Bin, Tan, Weimin, Yan, Bo, Wang, Shangfeng, Zhang, Fan

Issue&Volume: 2025-08-27

Abstract: Multiplexed fluorescence imaging provides valuable biological insights from the cellular to the tissue level but remains limited in live-mammal studies by the lack of a fluorescent palette capable of overcoming photon scattering and autofluorescence noise for real-time, multiplexed in vivo imaging. Here we present a fluorophore palette engineered from erbium(III)-phthalocyanine complexes, termed the lanthanide rainbow (Lanbow), which offers tunable near-infrared absorption and a unified 1,530nm emission with brightness surpassing that of existing molecular dyes. Lanbow uses excitation-encoded and efficient single-band detection in the 1,500–1,900nm shortwave infrared subregion, where tissue scattering and autofluorescence are minimized, enabling up to nine-colour imaging in deep tissues. We also demonstrate fluorescence-guided surgery featuring multiparametric anatomical identification and functional assessment, with deep-learning networks automating real-time analysis for intraoperative guidance. This study establishes a transformative platform for real-time, highly multiplexed imaging in live mammals.

DOI: 10.1038/s41566-025-01736-8

Source: https://www.nature.com/articles/s41566-025-01736-8