课题组研究人员提出立体细胞，一个基于高密度DNA纳米球（DNB）模式阵列的空间增强分辨率单细胞测序平台，它可以在宽输入范围内实现可扩展和无偏的细胞捕获，并支持高保真转录组分析。立体细胞进一步允许整合基于成像的模式和多组学策略，包括免疫荧光和表位分析。该平台还兼容分析细胞外囊泡、微结构和大细胞，而其空间分辨率有助于细胞-细胞相互作用、细胞微环境和亚细胞转录物定位的原位分析。总之，Stereo-cell为扩展单细胞研究应用提供了一个灵活的框架。
据悉，单细胞测序技术提高了人们对细胞异质性和生物复杂性的理解。然而，现有的方法在吞吐量、捕获均匀性、单元大小灵活性和技术可扩展性方面存在局限性。
附：英文原文
Title: Stereo-cell: Spatial enhanced-resolution single-cell sequencing with high-density DNA nanoball-patterned arrays
Author: Sha Liao, Xiaoxi Zhou, Chuanyu Liu, Chang Liu, Shijie Hao, Hongyu Luo, Huan Hou, Qian Liu, Zhe Zhang, Liyun Xiao, Yuan Xu, Yaling Huang, Sining Zhou, Xuerong Li, Yang Wang, Lulin Xie, Zhichun Zhou, Shichen Dong, Yiru Wang, Xiaojing Xu, Pengcheng Guo, Xiumei Lin, Jiajie Lei, Qiaoling Wang, Yuxin Gong, Jiaming Cheng, Zixin Yuan, Yongqing Yang, Zhi Huang, Shenglong Li, Yuhui Zheng, Shichen Yang, Xin Huang, Weiqing Liu, Mei Li, Zhonghan Deng, Xinyu Yang, Jianhua Yin, Yingjie Luo, Yiwei Lai, Yue Yuan, Mengnan Cheng, Bo Wang, Jiansong Ji, Miguel A. Esteban, Yuxiang Li, Ying Gu, Yijun Ruan, Liang Chen, Xiangdong Wang, Jun Xie, Jian Wang, Longqi Liu, Ao Chen, Xun Xu
Issue&Volume: 2025-08-21
Abstract: Single-cell sequencing technologies have advanced our understanding of cellular heterogeneity and biological complexity. However, existing methods face limitations in throughput, capture uniformity, cell size flexibility, and technical extensibility. We present Stereo-cell, a spatial enhanced-resolution single-cell sequencing platform based on high-density DNA nanoball (DNB)–patterned arrays, which enables scalable and unbiased cell capture at a wide input range and supports high-fidelity transcriptome profiling. Stereo-cell further allows integration with imaging-based modalities and multiomics strategies, including immunofluorescence and epitope profiling. This platform is also compatible with profiling extracellular vesicles, microstructures, and large cells, whereas its spatial resolution facilitates in situ analysis of cell-cell interactions, cellular microenvironments, and subcellular transcript localization. Together, Stereo-cell provides a flexible framework for expanding single-cell research applications.
DOI: adr0475
Source: https://www.science.org/doi/10.1126/science.adr0475