印度海得拉巴塔塔基础研究所Tamal Das小组的最新研究探明了边缘曲率驱动内质网重组并决定上皮迁移模式。2025年8月18日出版的《自然—细胞生物学》杂志发表了这项成果。

在这里，该课题组进行了细胞内绘图，以揭示在微图案和自然产生的间隙中，内质网（ER）都经历了边缘曲率依赖的形态重组，在凸边缘形成小管，在凹边缘形成片状。这种重组依赖于细胞骨架产生的突起和收缩力。数学模型表明，这些形态在各自的几何形态下使应变能最小化。在功能上，位于凸缘的内质网小管有利于垂直定向的局灶粘连，支持板足爬行；而位于凹缘的内质网片有利于平行定向的局灶粘连，支持钱包弦状运动。总之，内质网作为一个中心机械换能器，整合来自细胞骨架网络的信号，协调两种正交的细胞迁移模式。

研究人员表示，从单细胞挤压到厘米大小的伤口，各种大小和几何形状的上皮间隙出现在生物体中。它们的闭包包括两种正交模式：凸边的板足爬行和凹边的钱包弦状运动。驱动这种依赖曲率的迁移的机制尚不清楚。

附：英文原文

Title: Edge curvature drives endoplasmic reticulum reorganization and dictates epithelial migration mode

Author: Rawal, Simran, Keshavanarayana, Pradeep, Manoj, Diya, Khuntia, Purnati, Banerjee, Sanak, Thurakkal, Basil, Marwaha, Rituraj, Spill, Fabian, Das, Tamal

Issue&Volume: 2025-08-18

Abstract: From single-cell extrusion to centimetre-sized wounds, epithelial gaps of various sizes and geometries appear across organisms. Their closure involves two orthogonal modes: lamellipodial crawling at convex edges and purse string-like movements at concave edges. The mechanisms driving this curvature-dependent migration remain unclear. Here we perform an intracellular cartography to reveal that in both micropatterned and naturally arising gaps, the endoplasmic reticulum (ER) undergoes edge curvature-dependent morphological reorganizations, forming tubules at convex edges and sheets at concave edges. This reorganization depends on cytoskeleton-generated protrusive and contractile forces. Mathematical modelling reveals that these morphologies minimize strain energy under their respective geometric regime. Functionally, ER tubules at the convex edge favour perpendicularly oriented focal adhesions, supporting lamellipodial crawling, while ER sheets at the concave edge favour parallelly oriented focal adhesions, supporting purse string-like movements. Altogether, ER emerges as a central mechanotransducer, integrating signals from cytoskeletal networks to orchestrate two orthogonal modes of cell migration.

DOI: 10.1038/s41556-025-01729-3

Source: https://www.nature.com/articles/s41556-025-01729-3