近日，上海交通大学李健团队实现了人工法尼醇环氧化酶能简明地合成巯基萜类化合物。相关论文于2025年8月14日发表在《科学》杂志上。

人们对法尼醇的头端和尾端双键进行了高效的不对称环氧化反应，极大地促进了萜类天然产物的合成。然而，缺乏对相对惰性的法尼醇内烯烃直接不对称环氧化的方法，对许多萜类化合物的合成提出了重大挑战。

研究组描述了一种环氧化酶的工程，该酶能够选择性地环氧化法尼醇的内烯烃，具有高的区域选择性和对映选择性。所得到的环氧化中间体已成功地应用于简化多种美罗萜类化合物的合成，在大多数情况下，将合成总步骤减少了一半以上。

附：英文原文

Title: Artificial farnesol epoxidase enables a concise synthesis of meroterpenoids

Author: Jinxin Wang, Yunpeng Yin, Quan Zhang, Wei-Dong Zhang, Jian Li

Issue&Volume: 2025-08-14

Abstract: Efficient asymmetric epoxidation reactions have been developed for both the head end and tail end double bonds of farnesol, greatly facilitating the synthesis of terpenoid natural products. However, the lack of methods for direct asymmetric epoxidation of the relatively inert internal alkene of farnesol has posed major challenges in the synthesis of many terpenoids. In this study, we describe the engineering of an epoxidase capable of selectively epoxidizing the internal alkene of farnesol with high regioselectivity and enantioselectivity. The resulting epoxidized intermediate has been successfully applied to simplify the synthesis of a variety of meroterpenoids, reducing the total number of synthetic steps by more than half in most cases.

DOI: adt2096

Source: https://www.science.org/doi/10.1126/science.adt2096