瑞士苏黎世大学Daniel Razansky团队研究了用于实时功能光声微血管成像的超宽带高密度聚合物球形阵列。2025年7月7日出版的《光：科学与应用》杂志发表了这项成果。

由于其独特的能力，捕获体积层析成像信息与一个单一的闪光，光电（OA）断层扫描最近证明了超快的成像速度，最终限制了超声飞行时间。该方法的可扩展性和可实现的空间分辨率受到目前用于OA信号检测的压电复合阵列的窄带宽的限制。

研究组首次实现了基于柔性聚偏氟乙烯（PVDF）薄膜的高密度球形阵列技术，其超宽带（0.3–40 MHz）传感单元面积达亚平方毫米级，相较于传统压电复合材料探头，该技术可实现实时多尺度三维成像，具备22–35微米空间分辨率、卓越的图像保真度，以及超出一个数量级的信噪比增强。

研究组进一步展示了五维（光谱，时间分辨，体积）成像能力，通过可视化快速刺激引起的小鼠脑氧合变化和对人体深层微血管进行实时功能血管造影。这种新技术利用了OA的真正潜力，实现了跨尺度快速生物动力学的定量高分辨率可视化。

附：英文原文

Title: Ultrawideband high density polymer-based spherical array for real-time functional optoacoustic micro-angiography

Author: Subochev, Pavel V., Den-Ben, Xos Lus, Chen, Zhenyue, Prudnikov, Maxim B., Vorobev, Vladimir A., Kurnikov, Alexey A., Orlova, Anna G., Postnikova, Anna S., Kharitonov, Alexey V., Proyavin, Mikhail D., Ovsyannikov, Roman I., Sanin, Anatoly G., Kirillin, Mikhail Y., Montero de Espinosa, Francisco, Turchin, Ilya V., Razansky, Daniel

Issue&Volume: 2025-07-07

Abstract: Owing to its unique ability to capture volumetric tomographic information with a single light flash, optoacoustic (OA) tomography has recently demonstrated ultrafast imaging speeds ultimately limited by the ultrasound time-of-flight. The method’s scalability and the achievable spatial resolution are yet limited by the narrow bandwidth of piezo-composite arrays currently employed for OA signal detection. Here we report on the first implementation of high-density spherical array technology based on flexible polyvinylidene difluoride films featuring ultrawideband (0.3–40MHz) submm2 area elements, thus enabling real-time multi-scale volumetric imaging with 22–35μm spatial resolution, superior image fidelity and over an order of magnitude signal-to-noise enhancement compared to piezo-composite equivalents. We further demonstrate five-dimensional (spectroscopic, time-resolved, volumetric) imaging capabilities by visualizing fast stimulus-evoked cerebral oxygenation changes in mice and performing real-time functional angiography of deep human micro-vasculature. The new technology thus leverages the true potential of OA for quantitative high-resolution visualization of rapid bio-dynamics across scales.

DOI: 10.1038/s41377-025-01894-y

Source: https://www.nature.com/articles/s41377-025-01894-y