Hartmut Schmidt研究小组在研究中取得进展。他们报道了谷氨酸能新皮质钮扣中递质释放的细胞内Ca²⁺敏感性。该研究于2025年7月3日发表于国际一流学术期刊《科学》杂志上。

在这项工作中，该研究团队研究了新皮质突触介导Syt1触发释放的机制。该课题组人员通过激光光解笼中的Ca²⁺，测量了第5层锥体神经元突触Ca²⁺释放的依赖性。释放物具有较高的Ca²⁺亲和力和正协同性。小脑浦肯野细胞突触的测量和动力学模型表明与Syt2触发释放相比存在实质性差异。他们的研究结果表明，Syt1控制释放机制在中等Ca²⁺浓度下具有高可靠性和高塑性可控性。

据了解，Synaptotagmin-1 （Syt1）和Syt2是触发大脑同步释放钙离子的主要钙（Ca²⁺）传感器。

附：英文原文

Title: The intracellular Ca2+ sensitivity of transmitter release in glutamatergic neocortical boutons

Author: Grit Bornschein, Simone Brachtendorf, Anja Reinert, Abdelmoneim Eshra, Robert Kraft, Johannes Hirrlinger, Jens Eilers, Stefan Hallermann, Hartmut Schmidt

Issue&Volume: 2025-07-03

Abstract: Synaptotagmin-1 (Syt1) and Syt2 are the main calcium (Ca2+) sensors triggering synchronous release in the brain. In this work, we studied the mechanisms mediating Syt1-triggered release from neocortical synapses. We measured the Ca2+ dependency of release in layer 5 pyramidal neuron synapses by laser photolysis of caged Ca2+. Release had high Ca2+ affinity and positive cooperativity. Measurements at cerebellar Purkinje cell synapses and kinetic models indicate substantial differences compared with Syt2-triggered release. Our results suggest that Syt1-controlled release machineries are optimized for high reliability at moderate Ca2+ elevations and high plastic controllability.

DOI: adp0870

Source: https://www.science.org/doi/10.1126/science.adp0870