结直肠癌免疫细胞群的高清晰度空间转录组学分析，这一成果由Sarah E. B. Taylor课题组经过不懈努力而取得。相关论文于2025年6月5日发表在《自然—遗传学》杂志上。

课题组研究人员引入了高清Visium空间转录组技术（Visium HD），并对福尔马林固定石蜡包埋的人类结直肠癌样本（n=5）进行了研究。研究人员展示了Visium HD的高灵敏度，单细胞尺度分辨率和空间精度，生成了CRC样本的高度精细的全转录组空间图谱。课题组研究人员通过与肿瘤和T细胞的相互作用，在不同的空间生态位中鉴定出转录组学上不同的巨噬细胞亚群，它们具有潜在的促肿瘤和抗肿瘤功能。原位基因表达分析验证了他们的发现，并定位了具有抗肿瘤特征的巨噬细胞附近的克隆扩增T细胞群。他们的研究展示了高分辨率空间技术在了解TME细胞相互作用方面的力量，并为更大规模的研究铺平了道路，这些研究将揭示CRC生物学的机制和生物标志物，改善诊断和疾病管理策略。

据介绍，全面了解结直肠癌（CRC）的细胞行为和对肿瘤微环境（TME）的反应仍然很困难。

附：英文原文

Title: High-definition spatial transcriptomic profiling of immune cell populations in colorectal cancer

Author: Oliveira, Michelli Faria de, Romero, Juan Pablo, Chung, Meii, Williams, Stephen R., Gottscho, Andrew D., Gupta, Anushka, Pilipauskas, Susan E., Mohabbat, Seayar, Raman, Nandhini, Sukovich, David J., Patterson, David M., Taylor, Sarah E. B.

Issue&Volume: 2025-06-05

Abstract: A comprehensive understanding of cellular behavior and response to the tumor microenvironment (TME) in colorectal cancer (CRC) remains elusive. Here, we introduce the high-definition Visium spatial transcriptomic technology (Visium HD) and investigate formalin-fixed paraffin-embedded human CRC samples (n=5). We demonstrate the high sensitivity, single-cell-scale resolution and spatial accuracy of Visium HD, generating a highly refined whole-transcriptome spatial profile of CRC samples. We identify transcriptomically distinct macrophage subpopulations in different spatial niches with potential pro-tumor and anti-tumor functions via interactions with tumor and T cells. In situ gene expression analysis validates our findings and localizes a clonally expanded T cell population close to macrophages with anti-tumor features. Our study demonstrates the power of high-resolution spatial technologies to understand cellular interactions in the TME and paves the way for larger studies that will unravel mechanisms and biomarkers of CRC biology, improving diagnosis and disease management strategies.

DOI: 10.1038/s41588-025-02193-3

Source: https://www.nature.com/articles/s41588-025-02193-3