南方科技大学胡宇慧课题组在研究中取得进展。他们提出了果蝇单细胞三维时空多组学图谱揭示了细胞类型分化的全景关键调节因子。2025年6月26日出版的《细胞》杂志发表了这项成果。
研究团队介绍了Flysta3D-v2，这是一个全面的多组学图谱，涵盖了模式生物果蝇从胚胎到蛹的发育寿命。他们的数据集包括三维单细胞空间转录组学、单细胞转录组学和单细胞染色质可及性信息。通过整合多模态数据，研究团队在整个生物体的硅三维模型中生成了不断发展的三维模型。课题组人员进一步构建了组织发育轨迹，揭示了细胞类型分化的详细概况。以中肠为重点，该课题组确定了参与中肠细胞类型调节的转录因子，并验证了exex是铜细胞发育的关键调节因子。这个广泛的图谱提供了丰富的抵抗力，并作为一个系统的平台，以超高时空分辨率集成单细胞数据来研究果蝇的发育。
据悉，多细胞生物的发育是一个高度复杂的过程，受到许多基因和途径在空间和时间上的严格调控。
附：英文原文
Title: A Drosophila single-cell 3D spatiotemporal multi-omics atlas unveils panoramic key regulators of cell-type differentiation
Author: Mingyue Wang, Qinan Hu, Zhencheng Tu, Lingshi Kong, Tengxiang Yu, Zihan Jia, Yuetian Wang, Jiajun Yao, Rong Xiang, Zhan Chen, Yan Zhao, Yanfei Zhou, Qing Ye, Kang Ouyang, Xianzhe Wang, Yinqi Bai, Zhenyu Yang, Hanxiang Wang, Yanru Wang, Hanxiang Jiang, Tao Yang, Jing Chen, Yunting Huang, Ni Yin, Wenyuan Mo, Wenfu Liang, Chang Liu, Xiumei Lin, Chuanyu Liu, Ying Gu, Wei Chen, Longqi Liu, Xun Xu, Yuhui Hu
Issue&Volume: 2025-06-26
Abstract: The development of a multicellular organism is a highly intricate process tightly regulated by numerous genes and pathways in both spatial and temporal manners. Here, we present Flysta3D-v2, a comprehensive multi-omics atlas of the model organism Drosophila spanning its developmental lifespan from embryo to pupa. Our datasets encompass 3D single-cell spatial transcriptomic, single-cell transcriptomic, and single-cell chromatin accessibility information. Through the integration of multimodal data, we generated developmentally continuous in silico 3D models of the entire organism. We further constructed tissue development trajectories that uncover the detailed profiles of cell-type differentiation. With a focus on the midgut, we identified transcription factors involved in midgut cell-type regulation and validated exex as a key regulator of copper cell development. This extensive atlas provides a rich resource and serves as a systematic platform for studying Drosophila development with integrated single-cell data at ultra-high spatiotemporal resolution.
DOI: 10.1016/j.cell.2025.05.047
Source: https://www.cell.com/cell/abstract/S0092-8674(25)00629-4