近日，新加坡国立大学黄志伟团队揭示了高阶涡旋光束增强MINFLUX纳米显微镜。相关论文于2025年5月6日发表在《光：科学与应用》杂志上。

最小光子通量（MINFLUX）纳米显微镜已成为超分辨率成像领域的一项革命性进步，能够在各种生物场景中进行前所未有的纳米级观测。在这项工作中，研究组首次提出，采用高阶涡旋光束可以显著提高MINFLUX的性能，超越了传统MINFLUX使用一阶涡旋光束的局限性。 

该理论分析表明，对于标准MINFLUX，高阶涡旋光束可以通过与它们的阶数相对应的因子来提高最大定位精度，在最佳条件下可以接近亚纳米尺度，对于光栅扫描MINFLUX来说，高阶涡流光束可以在保持更高精度的同时提供更宽的视场。这些发现强调了高阶涡旋光束提高MINFLUX性能的潜力，为广泛应用的超高分辨率成像铺平了道路。

附：英文原文

Title: MINFLUX nanoscopy enhanced with high-order vortex beams

Author: Tan, Xiao-Jie, Huang, Zhiwei

Issue&Volume: 2025-05-06

Abstract: Minimal photon fluxes (MINFLUX) nanoscopy has emerged as a transformative advancement in superresolution imaging, enabling unprecedented nanoscale observations across diverse biological scenarios. In this work, we propose, for the first time, that employing high-order vortex beams can significantly enhance the performance of MINFLUX, surpassing the limitations of the conventional MINFLUX using the first-order vortex beam. Our theoretical analysis indicates that, for standard MINFLUX, high-order vortex beams can improve the maximum localization precision by a factor corresponding to their order, which can approach a sub-nanometer scale under optimal conditions, and for raster scan MINFLUX, high-order vortex beams allow for a wider field of view while maintaining enhanced precision. These findings underscore the potential of high-order vortex beams to elevate the performance of MINFLUX, paving the way towards ultra-high resolution imaging for a broad range of applications.

DOI: 10.1038/s41377-025-01822-0

Source: https://www.nature.com/articles/s41377-025-01822-0