北京大学第三医院杨睿团队取得一项新突破。他们提出了内窥镜妇科手术中体外成熟卵母细胞的DNA甲基化景观。该研究于2025年5月14日发表于国际一流学术期刊《遗传学报》杂志上。

人卵母细胞体外成熟（IVM）具有成本效益和微创性，是辅助生殖中保存生育能力、预防卵巢过度刺激综合征和其他生殖策略的有价值的补充工具。尽管IVM已有30年的历史，但由于疗效和安全性问题，其临床主题仍然有限。本研究通过亚硫酸盐还原测序检测了腹腔镜/宫腔镜手术中收集的IVM卵母细胞与体内成熟卵母细胞的DNA甲基化谱。结果表明，IVM卵母细胞表现出更高的整体甲基化水平。差异甲基化区（DMR）分析显示，与体内组相比，体外组显示出更多的高甲基化和更少的低甲基化DMR。此外，体外组的非CpG甲基化水平高于体内组。

然而，在这些卵母细胞中，甲基化水平与转录活性之间没有明显的相关性，特别是那些特定的印迹基因或与胚胎发育相关的基因。这些发现揭示了体外受精卵母细胞的表观遗传学景观，有助于持续评估其在辅助生殖中的临床可行性和安全性。

附：英文原文

Title: DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

Author: Cui-Ling Lu a b, Rui Yang b

Issue&Volume: 2025/05/14

Abstract: In vitro maturation (IVM) of human oocytes offers cost efficiency and minimal invasiveness, serving as a valuable supplementary tool in assisted reproduction for fertility preservation, ovarian hyperstimulation syndrome prevention, and other reproductive strategies. Despite its availability for three decades, the clinical use of IVM remains limited due to efficacy and safety concerns. This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing. Results indicate IVM oocytes exhibit a higher global methylation level. Differentially methylated regions (DMR) analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group. Additionally, the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group. However, no significant correlation between methylation levels and transcriptional activity in these oocytes is found, especially for those specific imprinted genes or genes related to embryonic development. These findings shed light on the epigenetic landscape of IVM oocytes, contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.

DOI: 10.1016/j.jgg.2025.05.002

Source: https://www.sciencedirect.com/science/article/abs/pii/S1673852725001444