低温电镜揭示天然RNA多价机制，这一成果由
该课题组人员使用低温电子显微镜（cryo-EM）来确定它们RNA家族的结构，包括ARRPOF和OLE形成二聚体，ROOL和gold形成六聚体，八聚体和十二聚体纳米结构，在2.6到4.6决议。这些同源寡聚体揭示了大量有助于RNA多价的结构基序，包括接吻环、回文碱基配对、a堆叠、金属离子配位、假结和小凹槽相互作用。这些结果提供了分子间相互作用驱动RNA多价与潜在功能相关性的分子基础。
据介绍，生物大分子的同源寡聚化导致功能组装，这对理解各种细胞过程至关重要。然而，RNA的四级结构很少被报道。比较基因组学分析已经确定了包含数百个序列的RNA家族，这些序列采用保守的二级结构，并可能折叠成复杂的三维（3D）结构。
附：英文原文
Title: Cryo-EM reveals mechanisms of natural RNA multivalency
Author: Liu Wang, Jiahao Xie, Tao Gong, Hao Wu, Yifan Tu, Xin Peng, Sitong Shang, Xinyu Jia, Haiyun Ma, Jian Zou, Sheng Xu, Xin Zheng, Dong Zhang, Yang Liu, Chong Zhang, Yongbo Luo, Zirui Huang, Bin Shao, Binwu Ying, Yu Cheng, Yingqiang Guo, Ying Lai, Dingming Huang, Jianquan Liu, Yuquan Wei, Siqi Sun, Xuedong Zhou, Zhaoming Su
Issue&Volume: 2025-03-13
Abstract: Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have been rarely reported. Comparative genomics analysis has identified RNA families containing hundreds of sequences that adopt conserved secondary structures and likely fold into complex three-dimensional (3D) structures. We use cryo-electron microscopy (cryo-EM) to determine structures from four RNA families, including ARRPOF and OLE forming dimers, and ROOL and GOLLD forming hexameric, octameric and dodecameric nanostructures, at 2.6 to 4.6 resolutions. These homo-oligomeric assemblies reveal a plethora of structural motifs that contribute to RNA multivalency, including kissing loop, palindromic base-pairing, A-stacking, metal ion coordination, pseudoknot and minor-groove interactions. These results provide the molecular basis of intermolecular interactions driving RNA multivalency with potential functional relevance.
DOI: adv3451
Source: https://www.science.org/doi/10.1126/science.adv3451