比利时根特大学Wim Derave研究团队通过对人体骨骼肌微环境的细胞解构，揭示了运动诱导的组胺能串联现象。相关论文于2025年2月6日发表在《细胞—代谢》杂志上。

利用对单个肌纤维进行转录组分析的工作流程，研究人员发现与大块肌肉组织反应相比，运动诱导的转录反应极其温和。通过整合单细胞数据，研究人员发现了一小部分肥大细胞，它们可能在运动过程中分泌组胺从而影响骨髓和血管细胞而非肌纤维细胞。

通过阻断人体组胺H1或H2受体，研究人员发现这种旁分泌型组胺信号级联可驱动肌糖原再合成并协调转录运动反应。总之，该研究通过对人体骨骼肌微环境的细胞解构揭示了组胺驱动的细胞间通讯网络，它调控肌肉恢复和对运动的适应。

据介绍，转录和翻译控制骨骼肌的可塑性以对运动做出响应，从而对健康和运动表现产生多重益处。骨骼肌微环境蕴藏着肌纤维和单核细胞，但在很大程度上，这种丰富的细胞多样性与运动适应性间的关系被忽视了。

附：英文原文

Title: Cellular deconstruction of the human skeletal muscle microenvironment identifies an exercise-induced histaminergic crosstalk

Author: Thibaux Van der Stede, Alexia Van de Loock, Guillermo Turiel, Camilla Hansen, Andrea Tamariz-Ellemann, Max Ullrich, Eline Lievens, Jan Spaas, Nurten Yigit, Jasper Anckaert, Justine Nuytens, Siegrid De Baere, Ruud Van Thienen, Anneleen Weyns, Laurie De Wilde, Peter Van Eenoo, Siska Croubels, John R. Halliwill, Pieter Mestdagh, Erik A. Richter, Lasse Gliemann, Ylva Hellsten, Jo Vandesompele, Katrien De Bock, Wim Derave

Issue&Volume: 2025-02-06

Abstract: Plasticity of skeletal muscle is induced by transcriptional and translational events in response to exercise, leading to multiple health and performance benefits. The skeletal muscle microenvironment harbors myofibers and mononuclear cells, but the rich cell diversity has been largely ignored in relation to exercise adaptations. Using our workflow of transcriptome profiling of individual myofibers, we observed that their exercise-induced transcriptional response was surprisingly modest compared with the bulk muscle tissue response. Through the integration of single-cell data, we identified a small mast cell population likely responsible for histamine secretion during exercise and for targeting myeloid and vascular cells rather than myofibers. We demonstrated through histamine H1 or H2 receptor blockade in humans that this paracrine histamine signaling cascade drives muscle glycogen resynthesis and coordinates the transcriptional exercise response. Altogether, our cellular deconstruction of the human skeletal muscle microenvironment uncovers a histamine-driven intercellular communication network steering muscle recovery and adaptation to exercise.

DOI: 10.1016/j.cmet.2024.12.011

Source: https://www.cell.com/cell-metabolism/abstract/S1550-4131(24)00493-5