德国欧洲分子生物学实验室Robert Prevedel研究团队，报道了基于成像傅里叶变换光谱仪的全场布里渊显微镜。相关论文于2025年2月20日发表在《自然—光子学》杂志上。
据介绍，布里温显微镜是一种新兴的光学弹性成像技术，可以以三维、全光学和非接触的方式评估生物样品的机械特性。然而，自发布里渊散射的低横截面产生微弱的信号，往往需要延长暴露时间或照射剂量，这对生物样品有潜在的危害。
本文提出了一种高度复用的布里渊散射光的快速光谱采集方法。具体来说，通过利用自制的傅里叶变换成像光谱仪和布里渊光谱的对称特性，该团队实验证明了幻觉和生物样品的二维光谱布里渊成像，吞吐量高达每秒40000个光谱，精度为~70MHz，有效的二维图像采集速度为0.1在~300× 300μm2视场。
与标准共聚焦方法相比，这在速度和吞吐量方面提高了大约三个数量级，同时保持了高空间分辨率，和获得生物学和医学中光敏样品三维图像的能力。
附：英文原文
Title: Full-field Brillouin microscopy based on an imaging Fourier-transform spectrometer
Author: Bevilacqua, Carlo, Prevedel, Robert
Issue&Volume: 2025-02-20
Abstract: Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a three-dimensional, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous Brillouin scattering produces weak signals that often necessitate prolonged exposure times or illumination dosages that are potentially harmful for biological samples. Here we present a new approach for highly multiplexed and therefore rapid spectral acquisition of the Brillouin-scattered light. Specifically, by exploiting a custom-built Fourier-transform imaging spectrometer and the symmetric properties of the Brillouin spectrum, we experimentally demonstrate full-field 2D spectral Brillouin imaging of phantoms as well as biological samples, at a throughput of up to 40,000 spectra per second, with a precision of ~70MHz and an effective 2D image acquisition speed of 0.1Hz over a ~300×300μm2 field of view. This represents an approximately three-orders-of-magnitude improvement in speed and throughput compared with standard confocal methods, while retaining high spatial resolution and the capability to acquire three-dimensional images of photosensitive samples in biology and medicine.
DOI: 10.1038/s41566-025-01619-y
Source: https://www.nature.com/articles/s41566-025-01619-y