巴黎萨克雷大学Charles-Antoine Dutertre课题组近日取得一项新成果。经过不懈努力，他们揭示了在人类肿瘤旁和恶性组织中DC亚群和状态。2025年12月8日，国际知名学术期刊《自然—免疫学》发表了这一成果。

为了生成无偏倚的人类DC图谱，该课题组研究人员整合了来自40个数据集的13个肿瘤组织的DC，以创建pDCmDC-VERSE （DC-VERSE）和mDC-VERSE单细胞RNA测序纲要。该课题组对这些组织中的DC亚群和“状态”进行了表征。大多数研究的肿瘤含有CD207⁺ DCs，这是CD1c⁺ DCs的一个亚群，其扩增与肿瘤CD8⁺常驻记忆T细胞、T细胞克隆和接受免疫检查点抑制剂治疗的患者的生存呈负相关。与CCR7⁺ mDCs（DC1s、DC2s和DC3s的共同状态）类似，研究人员发现CD207⁺ DCs是DC2s和DC3s的共同状态。空间分辨的单细胞转录组学和免疫组织荧光分析显示，淋巴细胞和大多数DC在肿瘤间质中富集，而CD207⁺ DCs大多包埋在肿瘤巢中。这些DC-VERSEs为科学界在健康和病理方面的DC提供了最有效的抵抗。

据悉，树突状细胞是一种专业的抗原呈递细胞。浆细胞样树突状细胞（pDCs）在稳态下是较差的抗原呈递细胞，而髓细胞样树突状细胞（mDCs），包括DC1s、DC2s和DC3s，专门用于T细胞启动。

附：英文原文

Title: DC subsets and states unraveled across human juxtatumoral and malignant tissues

Author: Mulder, Kevin, Gardet, Margaux, Kong, Wan Ting, Patel, Amit Ashok, Calvez, Anne, Gessain, Grgoire, de la Calle-Fabregat, Carlos, Piot, Ccile, Blampey, Quentin, Poupaud, Elisa, Anzali, Ahmed-Amine, Dunsmore, Garett, Bougouin, Antoine, Pupier, Guilhem, He, Lizhe, Wiggins, Timothy, He, Jiang, Emanuel, George, Goubet, Anne-Galle, Al-Eryani, Ghamdan, Swarbrick, Alexander, Michels, Judith, Dress, Regine J., Deloger, Marc, Bertoletti, Antonio, Thomas de Montpreville, Vincent, Sauts-Fridman, Catherine, Fridman, Wolf H., Zitvogel, Laurence, Ginhoux, Florent, Dutertre, Charles-Antoine

Issue&Volume: 2025-12-08

Abstract: Dendritic cells (DCs) are professional antigen-presenting cells. While plasmacytoid DCs (pDCs) are poor antigen-presenting cells at steady state, myeloid DCs (mDCs), which include DC1s, DC2s and DC3s, are specialized in T cell priming. To generate unbiased human DC atlases, we integrated DCs from 13 tumor tissues across 40 datasets to create a pDC+mDC-VERSE (DC-VERSE) and an mDC-VERSE single-cell RNA-sequencing compendium. We characterized DC subsets and ‘states’ across these tissues. Most studied tumors contained CD207+ DCs, a subset of CD1c+ DCs, whose expansion inversely correlated with tumor CD8+ resident memory T cells, T cell clonality and the survival of patients treated with immune checkpoint inhibitors. Similarly to CCR7+ mDCs (a common state of DC1s, DC2s and DC3s), we found that CD207+ DCs were a common state of DC2s and DC3s. Spatially resolved single-cell transcriptomic and immunohistofluorescence analyses of human carcinomas demonstrated that lymphocytes and most DCs were enriched within the tumor stroma, while CD207+ DCs were mostly embedded within tumor nests. These DC-VERSEs provide a robust resource available to the scientific community on DCs in health and pathology.

DOI: 10.1038/s41590-025-02337-x

Source: https://www.nature.com/articles/s41590-025-02337-x