在干细胞衍生的猴胚胎模型中模拟晚期原肠胚形成，这一成果由中国科学院脑科学与智能技术卓越创新中心刘真课题组经过不懈努力而取得。该项研究成果发表在2025年12月3日出版的《自然》上。

通过优化的3D胚泡培养系统，研究组成功地将干细胞衍生的猴胚泡体外培养提前到了第25天。形态学和组织学分析表明，这些猴胚状体经历了原肠胚形成，并在体内大致再现了原肠胚形成后期的关键发育事件，包括神经板、造血系统、尿囊、原始肠道、原始生殖细胞、卵黄囊结构和其他器官的祖细胞，但滋养细胞衍生物除外。单细胞转录组学分析显示，这些猴胚的细胞谱系组成和分化轨迹与自然胚胎在原肠胚形成过程中发现的相似。因此，这种灵长类干细胞衍生的胚胎模型为揭示灵长类动物胚胎从囊胚到原肠胚后期的发育机制提供了一个有价值的平台。

据介绍，干细胞衍生的胚胎模型可以极大地促进他们对胚胎发育的理解。虽然人类和猴子的胚胎模型已经达到了早期原肠胚阶段，但机器人模型的发育仍有待完成。

附：英文原文

Title: Modelling late gastrulation in stem cell-derived monkey embryo models

Author: Li, Jie, Li, Jie, Cao, Jing, Shang, ShenShen, Zhang, Liansheng, Gao, Fei, Fu, Jiqiang, Chen, Hongyu, Cui, Guizhong, Wu, Haoyuan, Wang, Xiaolong, Martinez-Arias, Alfonso, Sun, Qiang, Liu, Zhen

Issue&Volume: 2025-12-03

Abstract: Stem cell-derived embryo models could greatly facilitate our understanding of embryonic development. Although human and monkey embryo models have reached early gastrulation stage1,2,3,4,5,6,7, the development of robust models beyond this time remains to be accomplished8. Here, using an optimized 3D suspension culture system, we have successfully advanced the in vitro culture of a stem cell-derived monkey blastoid to day 25. Morphological and histological analyses showed that these monkey embryoids underwent gastrulation and largely recapitulated key developmental events of the late gastrulation stage observed in vivo, with the appearance of a neural plate, haematopoietic system, allantois, primitive gut, primordial germ cells, yolk sac structures and progenitors of other organs, excluding trophoblast derivatives. Single-cell transcriptomic analyses revealed that the lineage composition and differentiation trajectories of cells in these monkey embryoids were similar to those found in natural embryos during gastrulation. Thus, this primate stem cell-derived embryo model provides a valuable platform for dissecting the mechanisms of primate embryonic development from blastocyst to late gastrulation stage.

DOI: 10.1038/s41586-025-09831-0

Source: https://www.nature.com/articles/s41586-025-09831-0