在这里，该课题组开发了带有两亲性凝胶包膜（CAGEs）的胶囊，它可以选择性地保留细胞和大量分析物，同时可以自由地接触培养基、酶和试剂。胶囊能够将活细胞培养与全基因组读数相结合，进行高通量多步检测。该研究组建立了CAGE DNA文库的条形码方法，并通过在CAGE中捕获数十种扩展克隆的转录组，将其应用于测量细胞中基因表达程序的持久性。CAGEs与多种酶促反应的兼容性将有助于扩大目前单细胞、高通量测量的曲目，并将其扩展到活细胞分析。
据了解，单细胞测序方法揭示细胞之间的自然和诱导变异。然而，许多功能基因组学方法需要多个步骤，这些步骤还不能扩展到高通量，包括对活细胞的测定。
附：英文原文
Title: Multistep genomics on single cells and live cultures in subnanoliter capsules
Author: Ignas Mazelis, Haoxiang Sun, Arpita Kulkarni, Theresa L. Torre, Allon M. Klein
Issue&Volume: 2025-12-18
Abstract: Single-cell sequencing methods uncover natural and induced variation between cells. Many functional genomic methods, however, require multiple steps that cannot yet be scaled to high throughput, including assays on living cells. Here we develop capsules with amphiphilic gel envelopes (CAGEs), which selectively retain cells and large analytes while being freely accessible to media, enzymes and reagents. Capsules enable high-throughput multistep assays combining live-cell culture with genome-wide readouts. We establish methods for barcoding CAGE DNA libraries, and apply them to measure persistence of gene expression programs in cells by capturing the transcriptomes of tens of thousands of expanding clones in CAGEs. The compatibility of CAGEs with diverse enzymatic reactions will facilitate the expansion of the current repertoire of single-cell, high-throughput measurements and their extension to live-cell assays.
DOI: ady7209
Source: https://www.science.org/doi/10.1126/science.ady7209