上海科技大学向阳飞课题组宣布他们提出了人类PSC衍生的类器官模型交感神经节发育及其与心脏的功能串扰。这一研究成果于2025年12月11日发表在国际顶尖学术期刊《细胞—干细胞》上。

研究组报告了一种将人类多能干细胞分化成类似于周围交感神经节的三维神经器官的方法，以自组织的方式产生神经节的神经元和胶质细胞。该研究团队开发了一个类器官系统，通过将人类交感神经节类器官（hSGOs）和心脏形成类器官结合，在交感神经节和它们的外围目标之一心脏之间构建功能连接。值得注意的是，该系统能够评估人类交感神经到心脏神经支配的信号控制（即神经生长因子[NGF]信号），并揭示交感神经和心脏神经谱系在共同发育过程中的相互影响。他们的研究为理解人类交感神经节的发育、它们与外周靶点的串扰以及相关疾病提供了一个生理学相关的平台。

据了解，交感神经节是神经系统的重要组成部分，它调节着不自主身体功能的各个方面。用三维（3D）类器官再现交感神经节的发育是具有挑战性的，并且尚未实现。

附：英文原文

Title: Human PSC-derived organoids model sympathetic ganglion development and its functional crosstalk with the heart

Author: Yantong Liu, Jinkui Zhu, Xiaoxiang Lu, Xuran Zhuang, Jianfeng Wei, Linlin Jiang, Wei Zhou, Wei Pang, Yao Yin, Ziling Chen, Yajing Cao, Qinzhi Zhang, Sisi Chen, Siyuan Chu, Xinrui Zhang, Yangfei Xiang

Issue&Volume: 2025-12-11

Abstract: The sympathetic ganglia are essential components of the nervous system that regulate various aspects of involuntary body functions. Recapitulating sympathetic ganglion development with three-dimensional (3D) organoids is challenging and has not been achieved. Here, we report a method to differentiate human pluripotent stem cells into 3D neural organoids that resemble peripheral sympathetic ganglia, producing both neurons and glial cells of the ganglia in a self-organized manner. We developed an organoid system to construct functional connections between the sympathetic ganglia and one of their peripheral targets, the heart, by fusing human sympathetic ganglion organoids (hSGOs) and heart-forming organoids. Notably, this system enables the evaluation of signaling controls (i.e., nerve growth factor [NGF] signaling) on human sympathetic-to-cardiac innervation and reveals the reciprocal impacts between the sympathetic and cardiac lineages during their co-development. Our study provides a physiologically relevant platform for understanding the development of human sympathetic ganglia, their crosstalk with peripheral targets, and related diseases.

DOI: 10.1016/j.stem.2025.11.003

Source: https://www.cell.com/cell-stem-cell/abstract/S1934-5909(25)00410-2