近日，阿根廷国家科学和技术调查委员会Andrés Zelcer团队研究了用于超分辨率荧光显微镜的开源亚纳米稳定系统。相关论文发表在2025年11月20日出版的《光：科学与应用》杂志上。

荧光纳米显微镜技术的最新进展已将分辨率提升至1-10纳米尺度，即使在拥挤的生物环境中也能直接观测单个分子。要实现这种精度，需要严格的样品漂移控制技术。MINFLUX和RASTMIN等基于固定样品在激发光场中定位的技术，必须通过主动漂移校正才能达到理论上的纳米级分辨率极限。

研究组提出了一种用于超分辨显微镜的主动稳定系统，可提供持续数小时的亚纳米级稳定精度。该系统采用简洁的光路设计，能作为独立模块加装到各类荧光显微镜上。他们还提供了开源控制软件，其用户友好图形界面可快速适配不同实验配置。通过在两套独立实验装置上进行的p-MINFLUX和RASTMIN测量，研究组验证了该稳定系统的适应性与性能：其测量精度达到理论克拉美-罗界，并在DNA折纸结构中成功解析出约10纳米的间距。

附：英文原文

Title: Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy

Author: Edorna, Florencia, Choque, Florencia D., Ferrari, Giovanni, Masullo, Luciano A., Zdakowski, Piotr, Acuna, Guillermo P., Tinnefeld, Philip, Szalai, Alan M., Lopez, Luca F., Zelcer, Andrs, Stefani, Fernando D.

Issue&Volume: 2025-11-20

Abstract: Recent advances in fluorescence nanoscopy have pushed resolution to the 1–10nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this level of precision requires rigorous sample drift control. Techniques such as MINFLUX and RASTMIN, which rely on keeping the sample fixed within an excitation pattern, demand active drift correction to achieve their theoretical nanometer-scale resolution limits. Here, we present an active stabilization system for super-resolution microscopy that delivers sub-nm precision for hours. Featuring a simple optical design, the system can be added as a separate module to any fluorescence microscope. We also provide an open-source control software including a user-friendly graphical interface readily adaptable to different setups. We demonstrate the adaptability and performance of the stabilization system with p-MINFLUX and RASTMIN measurements performed in two different setups, reaching the theoretical Cramér-Rao Bound and resolving ~10nm distances within DNA origami structures.

DOI: 10.1038/s41377-025-02022-6

Source: https://www.nature.com/articles/s41377-025-02022-6