近日，北京师范大学曹玮团队报道了通过受控的自我固定脱膜来提高辐射反应性。2025年11月20日出版的《德国应用化学》杂志发表了这一最新研究成果。

通过液-液相分离（LLPS）形成的凝聚层已被用于模拟生物系统中无膜细胞器的动态组织。这些结构在生物医学领域展现出广阔的应用前景，尤其可作为生物化学反应的微反应器。然而，无膜凝聚层在环境条件下易发生聚结，且难以实现精确的刺激响应性释放，这为其生物医学应用带来重大挑战。

为解决该问题，研究组开发出自毁聚合物（SIP）膜包覆的凝聚层体系，可利用放射治疗γ射线调控酶促级联反应动力学。该SIP膜包覆的凝聚层表现出增强的动力学稳定性和抗融合性。在辐射作用下，SIP膜发生解聚反应，导致体系流动性增加并增强跨膜传输，进而调控凝聚层内的酶促级联反应进程。具体而言，研究组利用辐射响应型凝聚层精确调控活细胞内一氧化氮（NO）的生成，并通过NO介导的放射增敏效应增强细胞毒性。该研究推动了辐射响应型LLPS体系的发展，为细胞生物工程及放射-化学联合治疗等创新应用开辟了新途径。

附：英文原文

Title: Radiation-Responsive Coacervates Through Controlled Self-Immolative Demembranization

Author: Lixia Liu, Yuqing Qiao, Chula Sa, Kangrui Xu, Jiadong Tang, Ziwei Zhang, Chen Wang, Wei Cao

Issue&Volume: 2025-11-20

Abstract: Coacervates formed through liquid–liquid phase separation (LLPS) have been utilized to emulate the dynamic organization of membraneless organelles in biological systems. These structures exhibit broad application prospects in biomedicine, especially as microreactors for biochemical reactions. However, membraneless coacervates tend to coalesce easily in ambient condition and lack the ability to achieve precise, stimulus-responsive release, which presents significant challenges for their biomedical applications. To address this, we developed a self-immolative polymer (SIP)-membranized coacervates to tune enzyme cascade kinetics using radiotherapeutic γ-ray. The SIP-membranized coacervates exhibited enhanced kinetic stability and fusion-resistance. When exposed to radiation, the SIP membrane undergoes depolymerization, resulting in increased fluidity and enhanced transmembrane transport. This, in turn, regulated enzyme cascade reactions within the coacervates. Specifically, we used radiation-responsive coacervates to precisely modulate the generation of NO in living cells and exploited NO-mediated radiosensitization to enhance cytotoxicity. Our findings advance the development of radiation-responsive LLPS constructs, and pave the way for innovative applications in cellular bioengineering, and combined radio-chemotherapy.

DOI: 10.1002/anie.202519473

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202519473