课题组研究人员在持续黑暗的情况下，对144个非主题脑进行了组织清除和全脑c-Fos免疫染色。时间序列分析揭示了单细胞分辨率下的全脑昼夜节律性，642个解剖学定义的区域中有79%在不同的昼夜节律阶段振荡，描绘了功能特化。体素分析进一步突出了不同的子区域，表明区域内复杂的时空协调。此外，大脑昼夜节律时间可以从全球c-Fos模式主题组学衍生的预测方法中准确推断出来。这个全脑昼夜节律图谱增强了他们对神经协调的理解，并为将一天中的时间信息整合到功能和药理学研究中提供了一个抵抗。
研究人员表示，哺乳动物的大脑包括许多具有不同功能的解剖区域，尽管它们之间存在广泛的联系。然而，自发神经活动如何在昼夜周期中跨区域协调仍然是未知的。
附：英文原文
Title: A whole-brain single-cell atlas of circadian neural activity in mice
Author: Katsunari Yamashita, Fukuaki L. Kinoshita, Shota Y. Yoshida, Katsuhiko Matsumoto, Tomoki T. Mitani, Hiroshi Fujishima, Yoichi Minami, Eiichi Morii, Rikuhiro G. Yamada, Seiji Okada, Hiroki R. Ueda
Issue&Volume: 2025-11-13
Abstract: The mammalian brain comprises numerous anatomical regions with distinct functions despite their extensive connectivity. However, how spontaneous neural activity is coordinated across regions over the circadian cycle remains elusive. We employed tissue clearing and whole-brain c-Fos immunostaining on 144 mouse brains collected over two days under constant darkness. Time-series analysis revealed brain-wide circadian rhythmicity at single-cell resolution, with 79% of the 642 anatomically defined regions oscillating in diverse circadian phases that delineate functional specializations. Voxel-wise analyses further highlighted distinct subregions, suggesting intricate spatiotemporal coordination within regions. Additionally, brain circadian time can be accurately inferred from global c-Fos patterns using omics-derived prediction methods. This whole-brain circadian atlas enhances our understanding of neural coordination and provides a resource for integrating time-of-day information into functional and pharmacological research.
DOI: aea3381
Source: https://www.science.org/doi/10.1126/science.aea3381