近日，中国科学院长春光学精密机械与物理研究所李备团队报道了时间分辨光子计数傅里叶变换微光谱学能够同时进行拉曼和荧光寿命成像。相关论文发表在2025年10月29日出版的《光：科学与应用》杂志上。

为克服时间门控拉曼光谱技术现存的光谱范围狭窄与分辨率不足等局限，并实现拉曼与荧光寿命图像的同步采集，研究组开发了傅里叶变换光子计数光谱平台。该平台将配备高精度线性电机平台的马赫-曾德尔干涉仪与光子计数雪崩二极管及时标采集系统相结合，通过以下方式实现光子分类：以激发激光重复周期50纳秒为步长，根据光子粗到达时间确定平台位置矩阵；同时以80皮秒精度记录相对于100皮秒激发脉冲的精细到达时间。

该仪器具有547皮秒时间分辨率，支持532纳米激发波长下-1000至10,000 cm^-1的宽拉曼位移范围，并实现0.05 cm^-1的高光谱分辨率。在实验验证中，研究组采用荧光涂层硅片与荧光塑料微球样本，观察到激光激发脉冲时间分辨率范围内出现的拉曼信号，而荧光信号则在后续时段占主导。实验结果证实该仪器能有效分离拉曼与荧光信号。

附：英文原文

Title: Time-resolved photon counting Fourier-transform micro-spectroscopy enables simultaneous Raman and fluorescence lifetime imaging

Author: Shang, Lindong, Bao, Xiaodong, Peng, Hao, Chen, Fuyuan, Wang, Yu, Liu, Kunxiang, Liang, Peng, Wang, Yuntong, Tang, Xusheng, Masia, Francesco, Langbein, Wolfgang, Li, Bei

Issue&Volume: 2025-10-29

Abstract: To address the current limitations of time-gated Raman spectroscopy, specifically its narrow spectral range and low spectral resolution, and simultaneously acquire Raman and fluorescence life-time images, we have developed a Fourier-transform photon counting spectroscopy platform. A Mach-Zehnder interferometer employing a high accuracy linear motor stage was combined with photon-counting avalanche diodes and time-tagged acquisition, allowing to sort photons into a matrix of stage positions determined using their coarse arrival time with 50ns steps of the excitation laser repetition period, and a fine arrival time of 80ps resolution relative to the excitation pulse of 100ps duration. The instrument achieves a time resolution of 547ps, a wide spectral range of 1000 to 10,000cm1 Raman shift from the excitation at 532nm wavelength, and a high spectral resolution of 0.05cm1. For experimental validation, we used fluorescently coated silicon wafers and fluorescent plastic microspheres. Raman signal was observed during the laser excitation pulse within the time-resolution, while fluorescence signals dominate afterwards. The results confirm that the instrument can effectively separate Raman and fluorescence signals.

DOI: 10.1038/s41377-025-02020-8

Source: https://www.nature.com/articles/s41377-025-02020-8