德克萨斯大学Michael R. Green课题组宣布他们的研究认为肿瘤免疫微环境的多模态空间表征确定弥漫性大B细胞淋巴瘤。相关论文于2025年10月21日发表于国际顶尖学术期刊《自然—遗传学》杂志上。

研究人员应用高度多路空间转录组学和蛋白质组学以及基因组图谱来表征78个DLBCL肿瘤的免疫微环境结构。该课题组人员定义了7个不同的细胞生态位，每个都具有独特的细胞组成、空间组织和细胞间通讯模式，这些模式与T细胞和肿瘤B细胞。其中，来自免疫特权部位的DLBCL表现出丰富的T细胞浸润到异位壁龛，免疫细胞与肿瘤B细胞和具有激活和效应功能的转录特征，表明它们可能是抗肿瘤免疫的启动物。因此，DLBCL免疫微环境的空间特征揭示了促进细胞-细胞相互作用的不同模式的细胞壁龛，从而促进了壁龛驻留肿瘤和免疫细胞的表型异质性。

据悉，大号B细胞淋巴瘤（DLBCL）是一种异质性恶性肿瘤，可发生在淋巴结或结外部位，包括免疫特权部位。

附：英文原文

Title: Multi-modal spatial characterization of tumor immune microenvironments identifies targetable inflammatory niches in diffuse large Bcell lymphoma

Author: Dai, Yibo, Kizhakeyil, Atish, Chihara, Dai, Li, Xubin, Liu, Yunhe, Sainz Zuniga, Tania Patricia, Wilson, Ashley, Henderson, Jared, Vibe, Daniil, Petrosyants, Arman, Jacobson, Connor, Sarachakov, Alexander, Nomie, Krystle, Kryukov, Kirill, Bagaev, Aleksander, Chauhan, Ayushi, Westin, Jason R., Flowers, Christopher R., Vega, Francisco, Wang, Linghua, Green, Michael R.

Issue&Volume: 2025-10-21

Abstract: Diffuse large Bcell lymphomas (DLBCLs) are a heterogeneous group of malignancies that can arise in lymph nodes or extranodal locations, including immune-privileged sites. Here, we applied highly multiplexed spatial transcriptomics and proteomics together with genomic profiling to characterize the immune microenvironment architecture of 78 DLBCL tumors. We define seven distinct cellular niches, each characterized by unique cellular compositions, spatial organizations and patterns of intercellular communication associated with niche-specific phenotypes of both Tcells and tumor Bcells. Among these, DLBCLs from immune-privileged sites showed abundant Tcell infiltration into diffuse niches, where immune cells were intermixed with tumor Bcells and bore transcriptional hallmarks of activation and effector function, suggesting that they may be primed for anti-tumor immunity. Spatial characterization of the DLBCL immune microenvironment, therefore, reveals cellular niches that foster divergent patterns of cell–cell interactions contributing to the phenotypic heterogeneity of both niche-resident tumor and immune cells.

DOI: 10.1038/s41588-025-02353-5

Source: https://www.nature.com/articles/s41588-025-02353-5