在这项研究中，课题组人员研究了RADA16对hiPSC-CMs移植大鼠心脏血管化、肌原纤维结构和电生理适应的影响。RADA16是一种临床批准的自组装肽，注射后形成纳米纤维。RADA16加速了hiPSC-CMs向成人样基因表达谱的转变，增强了肌节组织，改善了移植部位的血管化。柔性网格纳米电子学揭示了移植的hiPSC-CMs在跳动的受体心脏内的颤动，RADA16显着降低了hiPSC-CMs的自动性。他们的发现证明了自组装纳米纤维在推进心脏细胞治疗方面的潜力，以及柔性网状纳米电子技术如何提高安全性。
据介绍，人类诱导多能干细胞来源的心肌细胞（hiPSC-CMs）的移植为心力衰竭提供了一种潜在的治疗方法，但移植细胞可能引起心律失常自动性。
附：英文原文
Title: Flexible nanoelectronics reveal arrhythmogenesis in transplanted human cardiomyocytes
Author: Junya Aoyama, Ren Liu, Xinhe Zhang, Anthony Y. Zhu, Pichayathida Luanpaisanon, Nivedhitha Velayutham, Jessica C. Garbern, Fang Cao, Irving Barrera, Hannah Fandl, Morgan Sokol, Satvik Dasariraju, Eun Seok Gil, Elton Aleksi, Toshi Amanuma, Jeffrey J. Saucerman, Fei Chen, Jia Liu, Richard T. Lee
Issue&Volume: 2025-10-16
Abstract: The transplantation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offers a potential treatment for heart failure, but arrhythmogenic automaticity arising from transplanted cells can arise. In this study, we investigated the effects of RADA16, a clinically approved self-assembling peptide that forms nanofibers after injection, on the vascularization, myofibril structure, and electrophysiological adaptation of hiPSC-CMs transplanted into rat hearts. RADA16 accelerated the transition of hiPSC-CMs toward adult-like gene expression profiles, enhanced sarcomere organization, and improved vascularization in the transplanted site. Flexible mesh nanoelectronics revealed fibrillation of transplanted hiPSC-CMs within the beating recipient heart, and RADA16 dramatically reduced the automaticity of hiPSC-CMs. Our findings demonstrate the potential of self-assembling nanofibers to advance cardiac cell therapy and how flexible mesh nanoelectronics technology could improve safety.
DOI: adw4612
Source: https://www.science.org/doi/10.1126/science.adw4612