该课题组人员开发了SalicS1，一种针对SA的基因编码荧光共振能量转移（FRET）生物传感器。SalicS1能够实时、可逆地监测体内SA水平，对内源性信号的干扰最小。该团队揭示了从细菌感染部位传播的SA激增的时空保真度。SalicS1解锁了对SA动态的精确理解，从而支持作物对病原体的抗性。
据了解，水杨酸（SA）是一种关键的植物激素，协调对病原体的免疫反应，包括丁香假单胞菌。SA积累的时间和程度受到植物的严格控制，但可以被病原体抑制以克服免疫。由于现有检测方法的局限性，在高时空分辨率下理解SA动态仍然具有挑战性，这些方法是间接的、破坏性的，或者缺乏细胞精度和时间分辨率。
附：英文原文
Title: SALICYLIC ACID SENSOR1 reveals the propagation of an SA hormone surge during plant pathogen advance
Author: Bijun Tang, Jing Lu, Hana Leontovyová, Gesa Hoffmann, James H. Rowe, Sacha Fouquay O’Donnell, Mathieu Grangé-Guermente, Bo Larsen, Rinukshi Wimalasekera, Philip Carella, Marco Incarbone, Tetiana Kalachova, Alexander M. Jones
Issue&Volume: 2025-10-09
Abstract: Salicylic acid (SA) is a key phytohormone that orchestrates immune responses against pathogens, including Pseudomonas syringae bacteria. The timing and extent of SA accumulation are tightly controlled by plants but can be suppressed by pathogens to overcome immunity. Understanding SA dynamics at high spatiotemporal resolution remains challenging owing to limitations in existing detection methods that are indirect, destructive, or lacking in cellular precision and temporal resolution. We developed SalicS1, a genetically encoded fluorescence resonance energy transfer (FRET) biosensor specific to SA. SalicS1 enables real-time, reversible monitoring of SA levels in vivo with minimal perturbation of endogenous signaling. We reveal the propagation of an SA surge spreading from bacterial infection sites with spatiotemporal fidelity. SalicS1 unlocks precise understanding of SA dynamics underpinning crop resilience to pathogens.
DOI: adw7650
Source: https://www.science.org/doi/10.1126/science.adw7650