该课题组研究人员比较了不同物种卵巢的三维成像、单细胞转录组学和功能研究。在小鼠中，该研究团队绘制了衰老过程中卵泡数量和卵母细胞能力下降的图谱；在人类卵巢中，该研究团队发现了皮质卵泡袋和密度下降。卵母细胞在生长过程中具有物种特异性的基因表达模式，趋同于成熟。不同物种的卵母细胞中年龄相关的转录变化大于颗粒细胞，尽管人类成熟卵母细胞的变化更多。课题组研究人员鉴定了卵巢交感神经和神经胶质；老年卵巢轴突密度增加，当在小鼠中切除时，卵泡发生受到干扰。这个比较图谱定义了卵巢生物学的共享和物种特异性标志。
据悉，母主题是人类卵巢生物学的一种易于处理的模型，然而，由于对转录和信号如何在特异性间和随年龄变化的不完全理解，其效用受到限制。
附：英文原文
Title: Comparative analysis of human and mouse ovaries across age
Author: Eliza A. Gaylord, Mariko H. Foecke, Ryan M. Samuel, Bikem Soygur, Angela M. Detweiler, Tara I. McIntyre, Leah C. Dorman, Michael Borja, Amy E. Laird, Ritwicq Arjyal, Juan Du, James M. Gardner, Norma Neff, Faranak Fattahi, Diana J. Laird
Issue&Volume: 2025-10-09
Abstract: The mouse is a tractable model for human ovarian biology, however its utility is limited by incomplete understanding of how transcription and signaling differ interspecifically and with age. We compared ovaries between species using three-dimensional imaging, single-cell transcriptomics, and functional studies. In mice, we mapped declining follicle numbers and oocyte competence during aging; in human ovaries, we identified cortical follicle pockets and decreases in density. Oocytes had species-specific gene expression patterns during growth that converged toward maturity. Age-related transcriptional changes were greater in oocytes than granulosa cells across species, although mature oocytes change more in humans. We identified ovarian sympathetic nerves and glia; axon density increased in aged ovaries and, when ablated in mice, perturbed folliculogenesis. This comparative atlas defines shared and species-specific hallmarks of ovarian biology.
DOI: adx0659
Source: https://www.science.org/doi/10.1126/science.adx0659