意大利技术研究院Ferruccio Pisanello等研究人员合作开发出，无标记和无报告器的最小侵入性拉曼光谱在小鼠大脑深部的应用。2024年12月31日，国际知名学术期刊《自然—方法学》在线发表了这一成果。

研究人员开发了振动光纤光度法，作为一种低侵入性的无标记方法，其通过自发拉曼光谱，在活体小鼠大脑深部任意区域监测生物分子内容。研究人员使用细至1微米的尖端锥形光纤探头，阐明小鼠大脑的细胞结构，监测由创伤性脑损伤引起的分子变化，并高精度地检测脑转移的标志物。

研究人员认为，该方法通过引入深度学习算法来抑制探头背景噪音，是对现有神经功能光学探测工具的有前景的补充，可应用于大脑及其他器官的基础研究和临床前研究。

据了解，光学方法监测神经活动正在改变神经科学，得益于快速发展的基因编码分子报告器的工具。然而，该领域仍需要强大且无标记的技术来监测，大脑发育、衰老或疾病过程中伴随的多方面生物分子变化。

附：英文原文

Title: Vibrational fiber photometry: label-free and reporter-free minimally invasive Raman spectroscopy deep in the mouse brain

Author: Pisano, Filippo, Masmudi-Martn, Mariam, Andriani, Maria Samuela, Cid, Elena, Kazemzadeh, Mohammadrahim, Pisanello, Marco, Balena, Antonio, Collard, Liam, Parras, Teresa Jurado, Bianco, Marco, Baena, Patricia, Tantussi, Francesco, Grande, Marco, Sileo, Leonardo, Gentile, Francesco, De Angelis, Francesco, De Vittorio, Massimo, Menendez de la Prida, Liset, Valiente, Manuel, Pisanello, Ferruccio

Issue&Volume: 2024-12-31

Abstract: Optical approaches to monitor neural activity are transforming neuroscience, owing to a fast-evolving palette of genetically encoded molecular reporters. However, the field still requires robust and label-free technologies to monitor the multifaceted biomolecular changes accompanying brain development, aging or disease. Here, we have developed vibrational fiber photometry as a low-invasive method for label-free monitoring of the biomolecular content of arbitrarily deep regions of the mouse brain in vivo through spontaneous Raman spectroscopy. Using a tapered fiber probe as thin as 1μm at its tip, we elucidate the cytoarchitecture of the mouse brain, monitor molecular alterations caused by traumatic brain injury, as well as detect markers of brain metastasis with high accuracy. We view our approach, which introduces a deep learning algorithm to suppress probe background, as a promising complement to the existing palette of tools for the optical interrogation of neural function, with application to fundamental and preclinical investigations of the brain and other organs.

DOI: 10.1038/s41592-024-02557-3

Source: https://www.nature.com/articles/s41592-024-02557-3