北京大学张泽民等研究人员合作发现，泛癌单细胞解剖揭示表型不同的B细胞亚型。相关论文于2024年7月23日在线发表在《细胞》杂志上。

研究人员通过整合来自649名患者的单细胞RNA测序数据，建立了涵盖19种主要癌症类型的人类B细胞综合资源。研究人员展示了肿瘤浸润B细胞（TIB）在总量和亚型组成上的显著异质性，并观察到抗体分泌细胞同种型的免疫球蛋白G（IgG）偏向。

此外，研究人员鉴定了应激反应记忆B细胞和肿瘤相关的非典型B细胞（TAAB），这两个在肿瘤中富集的亚群具有预后潜力，并在泛癌症中共享。特别是TAAB，具有高克隆扩展水平和增殖能力，并与肿瘤中的活化CD4 T细胞密切相互作用，能够预测免疫治疗的反应。该综合资源描绘了不同的临床相关TIB亚群，为进一步探索B细胞在癌症中的功能共性和多样性奠定了基础。

据悉，表征TIB的组成和表型特征对于推进人们对其在癌症发展中的作用的理解至关重要。

附：英文原文

Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes

Author: Yu Yang, Xueyan Chen, Jieying Pan, Huiheng Ning, Yaojun Zhang, Yufei Bo, Xianwen Ren, Jiesheng Li, Shishang Qin, Dongfang Wang, Min-Min Chen, Zemin Zhang

Issue&Volume: 2024-07-23

Abstract: Characterizing the compositional and phenotypic characteristics of tumor-infiltrating B cells (TIBs) is important for advancing our understanding of their role in cancer development. Here, we establish a comprehensive resource of human B cells by integrating single-cell RNA sequencing data of B cells from 649 patients across 19 major cancer types. We demonstrate substantial heterogeneity in their total abundance and subtype composition and observe immunoglobulin G (IgG)-skewness of antibody-secreting cell isotypes. Moreover, we identify stress-response memory B cells and tumor-associated atypical B cells (TAABs), two tumor-enriched subpopulations with prognostic potential, shared in a pan-cancer manner. In particular, TAABs, characterized by a high clonal expansion level and proliferative capacity as well as by close interactions with activated CD4 T cells in tumors, are predictive of immunotherapy response. Our integrative resource depicts distinct clinically relevant TIB subsets, laying a foundation for further exploration of functional commonality and diversity of B cells in cancer.

DOI: 10.1016/j.cell.2024.06.038

Source: https://www.cell.com/cell/fulltext/S0092-8674(24)00712-8