美国加州大学伯克利分校Na Ji研究团队完成突触传递的高通量容积映射。2024年6月19日，国际知名学术期刊《自然—方法学》在线发表了这一成果。

据介绍，体内突触传递的容积成像需要高空间分辨率和高时间分辨率。

附：英文原文

Title: High-throughput volumetric mapping of synaptic transmission

Author: Chen, Wei, Ge, Xinxin, Zhang, Qinrong, Natan, Ryan G., Fan, Jiang Lan, Scanziani, Massimo, Ji, Na

Issue&Volume: 2024-06-19

Abstract: Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at>1,000 synapses per volume and>500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.

DOI: 10.1038/s41592-024-02309-3

Source: https://www.nature.com/articles/s41592-024-02309-3