近日，华中农业大学殷平等研究人员合作揭示
研究人员表示，昆虫利用气味受体（OR）检测和分辨各种化学物质，气味受体是配体门控离子通道，由不同的气味传感OR和保守的气味受体共受体（Orco）组成。
研究人员报告了豌豆蚜Acyrthosiphon pisum的ApOR5-Orco异源复合体的单独结构以及与其已知激活配体乙酸香叶酯结合的结构。在这些结构中，三个ApOrco亚基作为支架成分，不能与配体结合，并保持相对不变。配体结合后，ApOR5的孔形成螺旋S7b从中心孔轴向外移动，导致离子流入的不对称孔开口。该研究深入揭示了OR-Orco异源复合体的气味识别和通道门控，并为开发用于害虫控制的创新型杀虫剂和驱虫剂提供了结构资源支持。
附：英文原文
Title: Structural basis for odorant recognition of the insect odorant receptor OR-Orco heterocomplex
Author: Yidong Wang, Liang Qiu, Bing Wang, Zeyuan Guan, Zhi Dong, Jie Zhang, Song Cao, Lulu Yang, Bo Wang, Zhou Gong, Liwei Zhang, Weihua Ma, Zhu Liu, Delin Zhang, Guirong Wang, Ping Yin
Issue&Volume: 2024-06-13
Abstract: Insects detect and discriminate a diverse array of chemicals using odorant receptors (ORs), which are ligand-gated ion channels comprising a divergent odorant-sensing OR and a conserved odorant receptor co-receptor (Orco). Here, we report structures of the ApOR5-Orco heterocomplex from the pea aphid Acyrthosiphon pisum alone and bound to its known activating ligand geranyl acetate. In these structures, three ApOrco subunits serve as scaffold components that cannot bind the ligand and remain relatively unchanged. Upon ligand binding, the pore-forming helix S7b of ApOR5 shifts outward from the central pore axis, causing an asymmetrical pore opening for ion influx. Our study provides insights into odorant recognition and channel gating of the OR-Orco heterocomplex and offers structural resources to support development of innovative insecticides and repellents for pest control.
DOI: adn6881
Source: https://www.science.org/doi/10.1126/science.adn6881