美国密歇根大学Kamran Diba团队发现，睡眠不足会降低海马的再激活和重放。相关论文于2024年6月12日在线发表在《自然》杂志上。

研究人员记录了大鼠在迷宫探索、睡眠和睡眠剥夺以及恢复睡眠期间12小时内的CA1神经元活动。研究人员发现，在睡眠剥夺期间，尖波涟漪（SWR）表现出持续或更高的速率，但功率较低，波纹频率较高。锥体细胞在睡眠剥夺期间表现出持续发射，而在睡眠期间发射减少，但无论睡眠状态如何，它们在SWR期间的发射率相当。尽管在睡眠剥夺期间，神经元会发生强烈的发射和大量的SWR，但研究人员发现，在这些时期，神经元发射模式的再激活和重放会减弱，在某些情况下，与自由睡眠相比，会完全消失。

恢复睡眠后，重新激活部分恢复，但未能达到自然睡眠时的水平。这些结果在网络水平上描述了睡眠丧失对海马功能的不利影响，并揭示了睡眠剥夺期间引起的许多SWR与这些事件期间发生的少数重新激活和重放之间的差异。

据了解，记忆得益于睡眠，而海马SWR期间清醒经验的重新激活和重放被认为是这一过程的关键。然而，人们对这些模式如何受到睡眠丧失的影响知之甚少。

附：英文原文

Title: Sleep loss diminishes hippocampal reactivation and replay

Author: Giri, Bapun, Kinsky, Nathaniel, Kaya, Utku, Maboudi, Kourosh, Abel, Ted, Diba, Kamran

Issue&Volume: 2024-06-12

Abstract: Memories benefit from sleep1, and the reactivation and replay of waking experiences during hippocampal sharp-wave ripples (SWRs) are considered to be crucial for this process2. However, little is known about how these patterns are impacted by sleep loss. Here we recorded CA1 neuronal activity over 12h in rats across maze exploration, sleep and sleep deprivation, followed by recovery sleep. We found that SWRs showed sustained or higher rates during sleep deprivation but with lower power and higher frequency ripples. Pyramidal cells exhibited sustained firing during sleep deprivation and reduced firing during sleep, yet their firing rates were comparable during SWRs regardless of sleep state. Despite the robust firing and abundance of SWRs during sleep deprivation, we found that the reactivation and replay of neuronal firing patterns was diminished during these periods and, in some cases, completely abolished compared to ad libitum sleep. Reactivation partially rebounded after recovery sleep but failed to reach the levels found in natural sleep. These results delineate the adverse consequences of sleep loss on hippocampal function at the network level and reveal a dissociation between the many SWRs elicited during sleep deprivation and the few reactivations and replays that occur during these events.

DOI: 10.1038/s41586-024-07538-2

Source: https://www.nature.com/articles/s41586-024-07538-2