上海交通大学杨洋团队报道了寡核苷酸通过具有特色路径设计的堆叠DNA纳米通道的行为。相关研究成果于2024年6月11日发表于国际一流学术期刊《美国化学会杂志》。

DNA纳米技术已成为构建穿透脂质双层人工通道的有用工具。

该文中，研究人员介绍了一种宽度可变的堆叠DNA折纸纳米通道设备，其由狭窄的入口和出口通道与宽的、可修改的管腔组成。这种设计调节了寡核苷酸的易位行为，揭示了记录的电流轨迹中不同阶段的信号模式。观察到的延长停留时间表明寡核苷酸保留，特别是由于从宽管腔到较窄出口通道的转变，而事件之间电流恢复的变化表明传导和阻断之间存在中间通道状态。

此外，通过在通道内腔中结合序列特异性悬突，研究人员在ATP适体易位事件中实现了独特的不对称电流分布。特色阶段还强调了适体结合动力学和ATP诱导的释放。具有内部装饰的堆叠DNA折纸通道所提供的独特的寡核苷酸，通过行为证明了DNA纳米通道工程在纳米器件开发和应用中的战略性和有益尝试。

附：英文原文

Title: Passing Behavior of Oligonucleotides through a Stacked DNA Nanochannel with Featured Path Design

Author: Rui Zhang, Yaozu Xiang, Yang Yang

Issue&Volume: June 11, 2024

Abstract: DNA nanotechnology has emerged as a useful tool for constructing artificial channels penetrating the lipid bilayer. In this work, we introduce a stacked DNA origami nanochannel device characterized by a width-variable pathway, consisting of narrow entrance and exit channels coupled with a wide, modifiable lumen. This design modulates the translocation behavior of oligonucleotides, revealing distinct stages of signal patterns in the recorded current traces. The observed prolonged dwell times indicate oligonucleotide retention, specifically due to the transition from the wide lumen to the narrower exit channel, while variations in current recovery between events suggested intermediate channel states between conducting and blocking. Further, by incorporating sequence-specific overhangs within the channel lumen, we achieved unique asymmetric current profiles during ATP aptamer translocation events. Featured stages also highlighted the aptamer binding dynamics and ATP-induced release. The distinguished oligonucleotide passing behaviors afforded by the stacked DNA origami channel with interior decoration demonstrated the strategic and profitable attempts at DNA nanochannel engineering for nanodevice development and applications.

DOI: 10.1021/jacs.4c02734

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.4c02734