复旦大学张琪团队表征了在细菌藿烷多元醇生物合成中作为嘌呤核苷磷酸化酶的HpnG。这一研究成果发表在2024年5月27日出版的国际学术期刊《中国化学》上。

据了解，HpnG在细菌藿烷多元醇生物合成的关键中间体——核糖藿烷的产生中起着至关重要的作用。尽管早期进行了广泛的研究，但HpnG的确切功能仍不清楚。

研究团队报道了HpnG作为嘌呤核苷磷酸化酶的功能表征，该酶在磷酸盐存在下将腺苷藿烷转化为磷酸核糖藿烷。HpnG具有广泛的底物特异性和令人印象深刻的稳定性，使其成为核苷加工和相关生物技术中有价值的酶促工具。

附：英文原文

Title: Characterization of HpnG as a Purine Nucleoside Phosphorylase in Bacteriohopanepolyol Biosynthesis†

Author: Xinhui Li, Xiaoyu Zhu, Yuting Zhong, Wei Zhang, Fener Chen, Wenning Wang, Wei Ding, Qi Zhang

Issue&Volume: 2024-05-27

Abstract: HpnG plays a crucial role in the production of ribosylhopane, a key intermediate in the biosynthesis of bacteriohopanepolyol. Despite early extensive studies, the precise function of HpnG has remained elusive. Here, we report functional characterization of HpnG as a purine nucleoside phosphorylase, which converts adenosylhopane to phosphoribosylhopane in the presence of phosphate. HpnG demonstrates broad substrate specificity and impressive stability, making it a valuable enzymatic tool for applications in nucleoside processing and related biotechnology.

DOI: 10.1002/cjoc.202400233

Source: https://onlinelibrary.wiley.com/doi/full/10.1002/cjoc.202400233