中国科学院遗传与发育生物学研究所Chuanyou Li和山东农业大学Lei Deng共同合作，近期取得重要工作进展。他们研究提出，肽REF1是促进植物再生的局部伤口信号。相关研究成果2024年5月22日在线发表于《细胞》杂志上。
据介绍，植物经常会受到伤害，并演化出非凡的再生能力来治愈伤口。然而，触发再生反应的伤口信号尚未确定。
通过对在创伤诱导的防御和再生中都有缺陷的番茄突变体的表征，研究人员证明，在番茄中，一种植物诱导肽（Pep），再生因子1（REF1），作为一种系统独立的局部创伤信号，主要调节局部防御反应和对创伤的再生反应。研究人员鉴定了PEPR1/2直接同源受体样激酶1（PORK1）是感知植物再生REF1信号的受体。
REF1-PORK1介导的信号传导通过激活创伤诱导的去分化1（WIND1）促进再生，WIND1是植物创伤诱导的细胞重编程的主要调节因子。因此，REF1-PORK1信号传导代表了一种保守的植物细胞因子途径，以启动、扩增和稳定信号级联，从而协调伤口触发的器官再生。REF1的应用为提高抗逆性作物的再生转化效率提供了一种简单的方法。
附：英文原文
Title: Peptide REF1 is a local wound signal promoting plant regeneration
Author: Wentao Yang, Huawei Zhai, Fangming Wu, Lei Deng, Yu Chao, Xianwen Meng, Qian Chen, Chenhuan Liu, Xiaomin Bie, Chuanlong Sun, Yang Yu, Xiaofei Zhang, Xiaoyue Zhang, Zeqian Chang, Min Xue, Yajie Zhao, Xiangbing Meng, Boshu Li, Xiansheng Zhang, Dajian Zhang, Xiangyu Zhao, Caixia Gao, Jiayang Li, Chuanyou Li
Issue&Volume: 2024-05-22
Abstract: Plants frequently encounter wounding and have evolved an extraordinary regenerative capacity to heal the wounds. However, the wound signal that triggers regenerative responses has not been identified. Here, through characterization of a tomato mutant defective in both wound-induced defense and regeneration, we demonstrate that in tomato, a plant elicitor peptide (Pep), REGENERATION FACTOR1 (REF1), acts as a systemin-independent local wound signal that primarily regulates local defense responses and regenerative responses in response to wounding. We further identified PEPR1/2 ORTHOLOG RECEPTOR-LIKE KINASE1 (PORK1) as the receptor perceiving REF1 signal for plant regeneration. REF1-PORK1-mediated signaling promotes regeneration via activating WOUND-INDUCED DEDIFFERENTIATION 1 (WIND1), a master regulator of wound-induced cellular reprogramming in plants. Thus, REF1-PORK1 signaling represents a conserved phytocytokine pathway to initiate, amplify, and stabilize a signaling cascade that orchestrates wound-triggered organ regeneration. Application of REF1 provides a simple method to boost the regeneration and transformation efficiency of recalcitrant crops.
DOI: 10.1016/j.cell.2024.04.040
Source: https://www.cell.com/cell/fulltext/S0092-8674(24)00466-5