日本大阪大学Katsumasa Fujita研究组开发出选择性平面激活结构照明显微镜。相关论文于2024年4月5日在线发表在《自然—方法学》杂志上。

研究人员利用选择性平面照明和可逆光开关荧光蛋白，实现了焦平面内的结构照明，消除了焦外背景。成像特性的理论研究和实验证明，选择性平面激活有利于细胞和细胞球体内致密微结构的成像。

据了解，结构照明显微镜在观察体积样品时，来自焦外平面的背景光会阻碍分辨率的提高。

附：英文原文

Title: Selective-plane-activation structured illumination microscopy

Author: Temma, Kenta, Oketani, Ryosuke, Kubo, Toshiki, Bando, Kazuki, Maeda, Shunsuke, Sugiura, Kazunori, Matsuda, Tomoki, Heintzmann, Rainer, Kaminishi, Tatsuya, Fukuda, Koki, Hamasaki, Maho, Nagai, Takeharu, Fujita, Katsumasa

Issue&Volume: 2024-04-05

Abstract: The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.

DOI: 10.1038/s41592-024-02236-3

Source: https://www.nature.com/articles/s41592-024-02236-3