南京大学医学院南京鼓楼医院肝胆外科赵远锦团队开发出了三维(3D)印迹细胞片的仿生肝小叶。相关论文于2024年2月28日发表于国际顶尖学术期刊《科学通报》杂志上。

他们提出了一种新的3D印迹细胞片策略，用于同步构建仿生肝微组织，在细胞类型，密度和分布方面具有高精度。为了实现这一目标，使用空间蛋白质基因组学数据集确定了正常人类肝脏中肝细胞的特定组成。数据和具有六边形空心截面的仿生肝微组织表明，使用自制的3d印迹设备，对肝细胞片进行逐层印迹和组装，成功地生成了细胞信息。

通过将血管内皮细胞浸润到组件的中空部分，可以获得具有血管化通道的仿生肝微组织，用于营养物质扩散和药物灌注。他们证明，由此产生的血管化的仿生肝脏微组织不仅可以集成到微流体药物筛选肝脏芯片中，还可以组装成一个扩大的生理结构，以促进肝脏再生。他们相信他们的3D印迹细胞片策略将为仿生微组织的构建开辟新的途径。

研究人员表示，肝组织工程已被证明在治疗肝脏疾病方面有价值，但构建高保真的肝组织仍然具有挑战性。

附：英文原文

Title: Biomimetic hepatic lobules from three-dimensional imprinted cell sheets

Author: anonymous

Issue&Volume: 2024/02/28

Abstract: Liver-tissue engineering has proven valuable in treating liver diseases, but the construction of liver tissues with high fidelity remains challenging. Here, we present a novel three-dimensional (3D)-imprinted cell-sheet strategy for the synchronous construction of biomimetic hepatic microtissues with high accuracy in terms of cell type, density, and distribution. To achieve this, the specific composition of hepatic cells in a normal human liver was determined using a spatial proteogenomics dataset. The data and biomimetic hepatic micro-tissues with hexagonal hollow cross-sections indicate that cell information was successfully generated using a homemade 3D-imprinted device for layer-by-layer imprinting and assembling the hepatic cell sheets. By infiltrating vascular endothelial cells into the hollow section of the assembly, biomimetic hepatic microtissues with vascularized channels for nutrient diffusion and drug perfusion can be obtained. We demonstrate that the resultant vascularized biomimetic hepatic micro-tissues can not only be integrated into a microfluidic drug-screening liver-on-a-chip but also assembled into an enlarged physiological structure to promote liver regeneration. We believe that our 3D-imprinted cell sheets strategy will open new avenues for biomimetic microtissue construction.

DOI: 10.1016/j.scib.2024.02.030

Source: https://www.sciencedirect.com/science/article/abs/pii/S2095927324001324