上海交通大学Chunhai Fan研究组近日取得一项新成果。经过不懈努力，他们研究出用于自旋过滤的扭曲DNA折纸基手性单层膜。相关论文于2024年2月26日发表在《美国化学会杂志》上。

在这项研究中，该课题组提出了一种制作高适应性扭曲DNA折纸手性单层的策略。这些结构具有明显的界面组装特征，有效地缓解了dsDNA单层结构的无序性，而dsDNA的持续长度限制在~50 nm。这一研究强调了七个代表性的DNA折纸手性单层膜的自旋过滤能力，与传统的dsDNA手性单层膜相比，每单位面积的自旋过滤效率最大增加了一个数量级。

有趣的是，他们的发现揭示了扭曲DNA折纸的高阶三级手性结构进一步提高了自旋过滤效率。这项工作为DNA手性单分子膜的合理设计铺平了道路。

据悉，具有固有手性的DNA单分子膜在生物传感器、DNA芯片和生物电子学等领域发挥着关键作用。然而，传统的DNA手性单层膜，通常由单链DNA (ssDNA)或双链DNA (dsDNA)构成，通常缺乏结构的有序性和界面设计的灵活性。结构DNA纳米技术已经成为解决这些挑战的一个有希望的解决方案。

附：英文原文

Title: Twisted DNA Origami-Based Chiral Monolayers for Spin Filtering

Author: Haozhi Wang, Fangfei Yin, Lingyun Li, Mingqiang Li, Zheng Fang, Chenyun Sun, Bochen Li, Jiye Shi, Jiang Li, Lihua Wang, Shiping Song, Xiaolei Zuo, Xiaoguo Liu, Chunhai Fan

Issue&Volume: February 26, 2024

Abstract: DNA monolayers with inherent chirality play a pivotal role across various domains including biosensors, DNA chips, and bioelectronics. Nonetheless, conventional DNA chiral monolayers, typically constructed from single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA), often lack structural orderliness and design flexibility at the interface. Structural DNA nanotechnology has emerged as a promising solution to tackle these challenges. In this study, we present a strategy for crafting highly adaptable twisted DNA origami-based chiral monolayers. These structures exhibit distinct interfacial assembly characteristics and effectively mitigate the structural disorder of dsDNA monolayers, which is constrained by a limited persistence length of ～50 nm of dsDNA. We highlight the spin-filtering capabilities of seven representative DNA origami-based chiral monolayers, demonstrating a maximal one-order-of-magnitude increase in spin-filtering efficiency per unit area compared with conventional dsDNA chiral monolayers. Intriguingly, our findings reveal that the higher-order tertiary chiral structure of twisted DNA origami further enhances the spin-filtering efficiency. This work paves the way for the rational design of DNA chiral monolayers.

DOI: 10.1021/jacs.3c11566

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.3c11566