南京中医药大学张毅楠团队报道了基于纳米NanoLuc-furimazine生物发光对的细菌硝基还原酶成像检测探针。相关研究成果于2024年2月23日发表在《中国化学》。

检测关键的内源性物种，如身体微环境中的细菌，需要更好的成像工具来可视化和监测生物事件。生物发光成像是活细胞和生物体中最流行的实时获取策略。

该文中，研究人员在furimazine的C-3位引入了硝基芳基，在C-6苯环引入了羟基，报道了第一个基于NanoLuc-furimazine生物发光对，用于检测细菌中硝基还原酶的生物发光探针（7），与其他生物发光同源物相比，具有最有效的去除效率，从而能够在一组临床优先病原体中高度选择性和敏感地显示NTR活性。此外，重组菌株以及来自小鼠粪便的NTR的成像表明了该探针在应用不同小鼠疾病模型中的潜力。

附：英文原文

Title: An Efficient Probe for Bacterial Nitroreductase Imaging and Detection Based on NanoLuc-Furimazine Bioluminescent Pair

Author: Ximeng Shi, Yumeng Wang, Jiaoyang Yu, Yating Yang, Zecheng Jin, Hanghang Wang, Dalei Wu, Wei Chen, Jianming Guo, Yinan Zhang

Issue&Volume: 2024-02-23

Abstract: The detection of critical endogenous species, such as bacteria in microenvironments in the body, requires better imaging tools for visualization and monitoring of biological events. Bioluminescence imaging is the most popular strategy for obtaining real-time in living cells and organisms. Herein, we introduced a nitroaryl group on the C-3 position and a hydroxy group at the C-6 phenyl ring on furimazine to report the first bioluminescent probe (7) based on NanoLuc-furimazine bioluminescent pair for the detection of nitroreductase in bacteria. The probe, which possessed up to 560-fold intensity increase with a low detection limit of 16 ng/mL of nitroreductase, has the most efficient uncage efficiency in comparison with other bioluminescent congeners, thus enabling highly selective and sensitive visualization of NTR activity in a panel of clinical priority pathogens. Additionally, imaging of the recombinant strain as well as the NTR from mouse feces indicated the potential of this probe in the application of different mouse disease models.

DOI: 10.1002/cjoc.202300702

Source: https://onlinelibrary.wiley.com/doi/full/10.1002/cjoc.202300702