中国科学院天津工业生物技术研究所张玲玲团队报道了重组类囊体膜整合的电驱动ATP合成。相关研究成果发表在2024年12月16日出版的《德国应用化学》。

大自然通过转换太阳能（光合磷酸化）和化学能（底物级磷酸化和氧化磷酸化）产生能量货币ATP。绿色电力作为一种重要的可持续能源载体，在实现碳中和社会中发挥着至关重要的作用。

该文中，研究人员建立并验证了一种新的电极驱动磷酸化方法。分离富含ATP酶的菠菜类囊体膜（TMs），并在质子交换膜（PEM）上构建平面TMs（pTMs），有效地赋予传统PEM ATP再生的生物学功能。

在优化了TMs浓度和孵育时间后，将生物PEM整合到一个两室电化学电池中，在电化学水分解过程中产生的质子梯度电位触发下，pTMs的ATP酶成功合成了ATP。

当向电化学电池施加3 V的恒定电压时，ATP的合成速率为3.16μM min^-1μgChl^-1，大约是ΔpH驱动的ATP合成速率的两倍。

该设计为体外生物转化（ivBT）系统中的绿色能源应用提供了巨大的潜力。

附：英文原文

Title: Electrodriven ATP Synthesis via Integration of a Reconstructed Thylakoid Membrane

Author: Lijing Chang, Huijuan Cui, Weisong Liu, Yi-Heng P. Job Zhang, Lingling Zhang

Issue&Volume: 2024-12-16

Abstract: Nature produces ATP, the energy currency, by converting solar energy (photophosphorylation) and chemical energy (substrate-level phosphorylation and oxidative phosphorylation). Green electricity, as a significant and sustainable energy carrier, plays a crucial role in achieving a carbon-neutral society. In this work, we established and verified a novel electrodriven phosphorylation method. Spinach thylakoid membranes (TMs), enriched with ATPases, were isolated and constructed into planar TMs (pTMs) on a proton exchange membrane (PEM), effectively imparting the traditional PEM with the biological function of ATP regeneration. Following the optimization of TMs concentration and incubation time, the biological PEM was integrated into a two-compartment electrochemical cell, where ATP was successfully synthesized by ATPase of pTMs, triggered by the proton gradient potential generated during electrochemical water splitting. When a constant voltage of 3 V was applied to the electrochemical cells, ATP was synthesized at a rate of 3.16 μM min-1μgChl-1, approximately twice the rate of ΔpH-driven ATP synthesis. This design offers substantial potential for green energy applications in in vitro biotransformation (ivBT) systems.

DOI: 10.1002/anie.202421120

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202421120