南京师范大学戴志晖团队报道了用于光声成像引导光热/基因治疗原位胶质母细胞瘤的简单炔基组装功能金纳米片。相关研究成果于2024年11月20日发表在《美国化学会杂志》。

由于存在血脑屏障（BBB）和肿瘤异质性，胶质母细胞瘤（GBM）的治疗仍然具有挑战性。

该文制备了用RGD肽和小干扰RNA（siRNA）功能化的Au纳米片（AuNSs），称为AuNSs-RGD-C≡C-iRNA（ARCR），以实现GBM的多模式治疗。使用设计良好的两亲性表面活性剂，可以制备出具有大可改性表面积、有趣的光热转换效率（50.26%）和显著的光热稳定性（7小时内44次循环）的AuNSs。

此外，炔基在1分钟内组装到Au表面上，使siRNA能够与AuNSs进行强共价结合，从而避免了生物硫醇的干扰。由于表面活性剂的亲脂性和RGD的靶向性，ARCR有效地穿过BBB并在GBM肿瘤区域积聚，从而使近红外光声成像引导光热/基因治疗。

该项工作提出了一种构建治疗性金基材料的简单策略，突出了多功能纳米药物在GBM治疗中的潜力。

附：英文原文

Title: Facile Alkyne Assembly-Enabled Functional Au Nanosheets for Photoacoustic Imaging-Guided Photothermal/Gene Therapy of Orthotopic Glioblastoma

Author: Xixi Hu, Peiling Li, Dongdong Xu, Hua Liu, Qiaoqiao Hao, Mengyang Zhang, Zhaoyin Wang, Tianxiang Wei, Zhihui Dai

Issue&Volume: November 20, 2024

Abstract: Treatment of glioblastoma (GBM) remains challenging due to the presence of blood–brain barrier (BBB) and tumor heterogeneity. Herein, Au nanosheets (AuNSs) functionalized with RGD peptides and small interfering RNA (siRNA), referred to as AuNSs-RGD-C≡C-siRNA (ARCR), are prepared to achieve multimodal therapy for GBM. The AuNSs with a large modifiable surface area, intriguing photothermal conversion efficiency (50.26%), and remarkable photothermal stability (44 cycles over 7 h) are created using a well-designed amphiphilic surfactant. Furthermore, alkynyl groups are assembled onto the Au surface within 1 min, enabling strong covalent binding of siRNA to AuNSs and thereby avoiding the interference from biological thiols. Owing to the lipophilicity of the surfactant and the targeting property of RGD, ARCR effectively passes through the BBB and accumulates in GBM tumor regions, allowing near-infrared photoacoustic imaging-guided photothermal/gene therapy. This work proposes a facile strategy to construct theranostic Au-based materials, highlighting the potential of multifunctional nanoagents for GBM therapy.

DOI: 10.1021/jacs.4c08990

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.4c08990