中国科学院动物研究所Hongmei Wang与北京干细胞与再生医学研究所Zhenyu Xiao等合作，近期取得重要工作进展。他们通过人类胎盘合胞体滋养层细胞的单核多组学分析鉴定了妊娠期间细胞的发育轨迹。相关研究成果2024年1月24日在线发表于《自然—遗传学》杂志上。

据介绍，人类胎盘在确保成功怀孕方面发挥着至关重要的作用。尽管对其细胞组成和功能的了解越来越多，但对合胞体滋养层（STB）内数十亿个细胞核的异质性的研究有限，STB是一种主要负责胎盘功能的多核实体。

研究人员对妊娠早期和晚期的人类胎盘进行了整合的单核RNA测序和单核ATAC测序分析。研究结果证明了STB细胞核的动态异质性和发育轨迹，以及它们与人类滋养层干细胞（hTSC）来源的STB的对应关系。

此外，研究人员通过转录因子的基因调控网络鉴定了与不同STB核谱系相关的转录因子，并通过实验证实了它们在hTSC和滋养层类器官来源STB中的功能。

总之，这一数据为人类STB的异质性提供了见解，并为解释相关妊娠并发症提供了宝贵的资源。

附：英文原文

Title: Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts identifies cellular trajectories during pregnancy

Author: Wang, Meijiao, Liu, Yawei, Sun, Run, Liu, Fenting, Li, Jiaqian, Yan, Long, Zhang, Jixiang, Xie, Xinwei, Li, Dongxu, Wang, Yiming, Li, Shiwen, Zhu, Xili, Li, Rong, Lu, Falong, Xiao, Zhenyu, Wang, Hongmei

Issue&Volume: 2024-01-24

Abstract: The human placenta has a vital role in ensuring a successful pregnancy. Despite the growing body of knowledge about its cellular compositions and functions, there has been limited research on the heterogeneity of the billions of nuclei within the syncytiotrophoblast (STB), a multinucleated entity primarily responsible for placental function. Here we conducted integrated single-nucleus RNA sequencing and single-nucleus ATAC sequencing analyses of human placentas from early and late pregnancy. Our findings demonstrate the dynamic heterogeneity and developmental trajectories of STB nuclei and their correspondence with human trophoblast stem cell (hTSC)-derived STB. Furthermore, we identified transcription factors associated with diverse STB nuclear lineages through their gene regulatory networks and experimentally confirmed their function in hTSC and trophoblast organoid-derived STBs. Together, our data provide insights into the heterogeneity of human STB and represent a valuable resource for interpreting associated pregnancy complications.

DOI: 10.1038/s41588-023-01647-w

Source: https://www.nature.com/articles/s41588-023-01647-w