美国洛克菲勒大学曹俊越研究团队报道，通过可扩展的单细胞RNA谱分析汇集的CRISPR筛选解剖转录组动力学的关键调节因子。2023年9月25日出版的《自然—生物技术》杂志发表了这项成果。

他们提出了一种组合索引方法，微扰Sci-Kinetics，在单细胞水平上跨越数百个遗传扰动以捕获整个转录组，新生转录组和单导RNA (sgRNA)身份。

通过分析针对各种生物过程的CRISPR筛选，他们展示了RNA合成、加工和降解、miRNA生物发生和线粒体mRNA加工过程中的基因表达调控，系统地解码了RNA时间动态基础的全基因组调控网络。

附：英文原文

Title: Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens

Author: Xu, Zihan, Sziraki, Andras, Lee, Jasper, Zhou, Wei, Cao, Junyue

Issue&Volume: 2023-09-25

Abstract: We present a combinatorial indexing method, PerturbSci-Kinetics, for capturing whole transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across hundreds of genetic perturbations at the single-cell level. Profiling a pooled CRISPR screen targeting various biological processes, we show the gene expression regulation during RNA synthesis, processing and degradation, miRNA biogenesis and mitochondrial mRNA processing, systematically decoding the genome-wide regulatory network that underlies RNA temporal dynamics at scale.

DOI: 10.1038/s41587-023-01948-9

Source: https://www.nature.com/articles/s41587-023-01948-9